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Characterization of the epidermal-dermal junction in hiPSC-derived skin organoids

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dc.contributor.authorRamovs, Veronika
dc.contributor.authorJanssen, Hans
dc.contributor.authorFuentes, Ignacia
dc.contributor.authorPitaval, Amandine
dc.contributor.authorRachidi, Walid
dc.contributor.authorChuva de Sousa Lopes, Susana M.
dc.contributor.authorFreund, Christian
dc.contributor.authorGidrol, Xavier
dc.contributor.authorMummery, Christine L.
dc.contributor.authorRaymond, Karine
dc.date.accessioned2023-07-07T20:02:07Z
dc.date.available2023-07-07T20:02:07Z
dc.date.issued2022
dc.description.abstractHuman induced pluripotent stem cell (hiPSC)-derived hair-bearing skin organoids offer exciting new possibilities for modeling diseases like epidermolysis bullosa (EB). These inherited diseases affect 1 in 30,000 people worldwide and result from perturbed expression and/or structure of components of the epidermal-dermal junction (EDJ). To establish whether hiPSC-derived skin organoids might be able to capture salient features of EB, it is thus important to characterize their EDJ. Here, we report successful generation of hair-bearing skin organoids from two hiPSC lines that exhibited fully stratified interfollicular epidermis. Using immunofluorescence and electron microscopy, we showed that basal keratinocytes in organoids adhere to laminin-332 and type IV collagen-rich basement membrane via type I hemidesmosomes and integrin β1-based adhesion complexes. Importantly, we demonstrated that EDJs in organoids are almost devoid of type VII collagen, a fibril that mediates anchorage of the epidermis to dermis. This should be considered when using skin organoids for EB modeling.
dc.description.versionVersión publicada
dc.format.extent10 p.
dc.identifier.citationStem Cell Reports. 2022 Jun 14;17(6):1279-1288
dc.identifier.doihttps://doi.org/10.1016/j.stemcr.2022.04.008
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7671
dc.language.isoen
dc.subjectCollagen
dc.subjectEpidermal-dermal junction
dc.subjecthiPSC-derived hair-bearing skin organoids
dc.titleCharacterization of the epidermal-dermal junction in hiPSC-derived skin organoids
dc.typeArticle
dcterms.accessRightsAcceso abierto
dspace.entity.typePublication

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