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The Autophagy Protein Pacer Positively Regulates the Therapeutic Potential of Mesenchymal Stem Cells in a Mouse Model of DSS-Induced Colitis

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dc.contributor.authorBergmann, Cristian
dc.contributor.authorBeltran, Sebastian
dc.contributor.authorVega, Ana
dc.contributor.authorMurgas, Paola
dc.contributor.authorHernández, Maria
dc.contributor.authorGómez, Laura
dc.contributor.authorLabrador, Luis
dc.contributor.authorCortés, Bastián
dc.contributor.authorPoblete, Cristian
dc.contributor.authorQuijada, Cristobal
dc.contributor.authorCarrión, Flavio
dc.contributor.authorWoehlbier, Ute
dc.contributor.authorManque, Patricio
dc.date.accessioned2023-07-07T14:50:46Z
dc.date.available2023-07-07T14:50:46Z
dc.date.issued2022
dc.description.abstractMesenchymal stem cells (MSC) have emerged as a promising tool to treat inflammatory diseases, such as inflammatory bowel disease (IBD), due to their immunoregulatory properties. Frequently, IBD is modeled in mice by using dextran sulfate sodium (DSS)-induced colitis. Recently, the modulation of autophagy in MSC has been suggested as a novel strategy to improve MSC-based immunotherapy. Hence, we investigated a possible role of Pacer, a novel autophagy enhancer, in regulating the immunosuppressive function of MSC in the context of DSS-induced colitis. We found that Pacer is upregulated upon stimulation with the pro-inflammatory cytokine TNFα, the main cytokine released in the inflammatory environment of IBD. By modulating Pacer expression in MSC, we found that Pacer plays an important role in regulating the autophagy pathway in this cell type in response to TNFα stimulation, as well as in regulating the immunosuppressive ability of MSC toward T-cell proliferation. Furthermore, increased expression of Pacer in MSC enhanced their ability to ameliorate the symptoms of DSS-induced colitis in mice. Our results support previous findings that autophagy regulates the therapeutic potential of MSC and suggest that the augmentation of autophagic capacity in MSC by increasing Pacer levels may have therapeutic implications for IBD.
dc.description.versionVersión Publicada
dc.identifier.citationBergmann CA, Beltran S, Vega-Letter AM, Murgas P, Hernandez MF, Gomez L, Labrador L, Cortés BI, Poblete C, Quijada C, Carrion F, Woehlbier U, Manque PA. The Autophagy Protein Pacer Positively Regulates the Therapeutic Potential of Mesenchymal Stem Cells in a Mouse Model of DSS-Induced Colitis. Cells. 2022 Apr 30;11(9):1503. doi: 10.3390/cells11091503
dc.identifier.doihttps://doi.org/10.3390/cells11091503
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7647
dc.language.isoen
dc.subjectKIAA0226L
dc.subjectAutophagy
dc.subjectPACER
dc.subjectRUBCNL
dc.subjectTherapy
dc.subjectInflammatory bowel disease
dc.subjectColitis
dc.subjectMesenchymal stem cells
dc.titleThe Autophagy Protein Pacer Positively Regulates the Therapeutic Potential of Mesenchymal Stem Cells in a Mouse Model of DSS-Induced Colitis
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceCells
dspace.entity.typePublication

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