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Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile

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dc.contributor.authorPorte, Lorena
dc.contributor.authorCruz, Rodrigo
dc.contributor.authorPérez, Inia
dc.contributor.authorVarela, Carmen
dc.contributor.authorDíaz, Cristina
dc.contributor.authorGarcía, Patricia
dc.contributor.authorLegarraga, Paulette
dc.contributor.authorValdivieso, Francisca
dc.contributor.authorWeitzel, Thomas
dc.date.accessioned2025-08-20T20:10:15Z
dc.date.available2025-08-20T20:10:15Z
dc.date.issued2024
dc.description.abstractFilamentous fungi are an emergent cause of severe infections in immunocompromised patients. Timely and accurate identification is crucial to initiate appropriate therapy. Traditional identification methods are time-consuming, labor-intensive, and operator-dependent. Matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry is a rapid and easy-to-perform identification method. The effectiveness of a commercial MALDI-TOF MS platform to identify filamentous fungi in a clinical laboratory was evaluated. The study included 67 fungal isolates from 35 species/species complexes, which were identified and confirmed in mycology reference laboratories; 32 derived from clinical samples, 34 from strain collections and one was an ATCC strain. The study used the VITEK® MS system (v3.2.0 database), after sample extraction by VITEK® MS Mould Kit. Results were classified as "correct species", "correct species complex", "correct genus"and "no identification". VITEK® MS correctly identified 91.0% of isolates (58.2% to species, 29.9% to species complex, and 1.5% to genus level only). In 82%, the result matched the species/species complex identified by reference methods. No misidentifications were observed. The kit was rapid and easy to use. In conclusion, the VITEK® MS system showed a high capability to accurately identify filamentous fungi in a clinical laboratory.
dc.description.versionVersión Publicada
dc.identifier.citationPorte L, Cruz R, Pérez I, Varela C, Díaz C, García P, Legarraga P, Valdivieso F, Weitzel T. Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. PLoS One. 2024 Dec 23;19(12):e0315690. doi: 10.1371/journal.pone.0315690
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0315690
dc.identifier.urihttps://hdl.handle.net/11447/10202
dc.language.isoen
dc.subjectLaboratories
dc.subjectFungi* / isolation & purification
dc.subjectMycoses / diagnosis
dc.subjectSpectrometry
dc.titlePerformance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourcePLoS One
dspace.entity.typePublication
relation.isAuthorOfPublicationc641ef0c-51cb-4d25-8e9f-c8139bc6a4ad
relation.isAuthorOfPublication.latestForDiscoveryc641ef0c-51cb-4d25-8e9f-c8139bc6a4ad

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