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Senescence Markers in Peripheral Blood Mononuclear Cells in Amnestic Mild Cognitive Impairment and Alzheimer’s Disease

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dc.contributor.authorSalech, Felipe
dc.contributor.authorSanMartín, Carol D.
dc.contributor.authorConcha-Cerda, Jorge
dc.contributor.authorPonce, Daniela P.
dc.contributor.authorLiabeuf, Gianella
dc.contributor.authorRogers, Nicole K.
dc.contributor.authorRomero-Hernández, Esteban
dc.contributor.authorMurgas, Paola
dc.contributor.authorBruna, Bárbara
dc.contributor.authorMore, Jamileth
dc.contributor.authorBehrens, María Isabel
dc.date.accessioned2023-07-07T16:27:29Z
dc.date.available2023-07-07T16:27:29Z
dc.date.issued2022
dc.description.abstractRecent studies suggest that cellular senescence plays a role in Alzheimer’s Disease (AD) pathogenesis. We hypothesize that cellular senescence markers might be tracked in the peripheral tissues of AD patients. Senescence hallmarks, including altered metabolism, cell-cycle arrest, DNA damage response (DDR) and senescence secretory associated phenotype (SASP), were measured in peripheral blood mononuclear cells (PBMCs) of healthy controls (HC), amnestic mild cognitive impairment (aMCI) and AD patients. Senescence-associated βeta-galactosidase (SA-β-Gal) activity, G0-G1 phase cell-cycle arrest, p16 and p53 were analyzed by flow cytometry, while IL-6 and IL-8 mRNA were analyzed by qPCR, and phosphorylated H2A histone family member X (γH2AX) was analyzed by immunofluorescence. Senescent cells in the brain tissue were determined with lipofuscin staining. An increase in the number of senescent cells was observed in the frontal cortex and hippocampus of advanced AD patients. PBMCs of aMCI patients, but not in AD, showed increased SA-β-Gal compared with HCs. aMCI PBMCs also had increased IL-6 and IL8 mRNA expression and number of cells arrested at G0-G1, which were absent in AD. Instead, AD PBMCs had significantly increased p16 and p53 expression and decreased γH2Ax activity compared with HC. This study reports that several markers of cellular senescence can be measured in PBMCs of aMCI and AD patients.
dc.description.versionVersión publicada
dc.format.extent15 p.
dc.identifier.citationInt. J. Mol. Sci. 2022, 23, 9387
dc.identifier.doihttps://doi.org/10.3390/ ijms23169387
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7658
dc.language.isoes
dc.subjectCellular senescence
dc.subjectPeripheral blood mononuclear cells
dc.subjectAlzheimer’s Disease
dc.subjectaMCI
dc.subjectAging
dc.titleSenescence Markers in Peripheral Blood Mononuclear Cells in Amnestic Mild Cognitive Impairment and Alzheimer’s Disease
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceInternational Journal of Molecular Sciences
dspace.entity.typePublication

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