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Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile

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dc.contributor.authorQuezada-Aguiluz, Mario
dc.contributor.authorAguayo-Reyes, Alejandro
dc.contributor.authorCarrasco, Cinthia
dc.contributor.authorMejías, Daniela
dc.contributor.authorSaavedra, Pamela
dc.contributor.authorMella-Montecinos, Sergio
dc.contributor.authorOpazo-Capurro, Andrés
dc.contributor.authorBello-Toledo, Helia
dc.contributor.authorMunita, José
dc.contributor.authorHormazábal, Juan C.
dc.contributor.authorGonzález-Rocha, Gerardo
dc.date.accessioned2023-07-07T17:32:19Z
dc.date.available2023-07-07T17:32:19Z
dc.date.issued2022
dc.description.abstractMacrolides, lincosamides, and type B streptogramins (MLSB) are important therapeutic options to treat methicillin-resistant Staphylococcus aureus (MRSA) infections; however, resistance to these antibiotics has been emerging. In Chile, data on the MLSB resistance phenotypes are scarce in both community-(CA) and hospital-acquired (HA) MRSA isolates. Antimicrobial susceptibility to MLSB was determined for sixty-eight non-repetitive isolates of each HA-(32) and CA-MRSA (36). Detection of SCCmec elements, ermA, ermB, ermC, and msrA genes was performed by PCR. The predominant clones were SCCmec I-ST5 (HA-MRSA) and type IVc-ST8 (CA-MRSA). Most of the HA-MRSA isolates (97%) showed resistance to clindamycin, erythromycin, azithromycin, and clarithromycin. Among CA-MRSA isolates, 28% were resistant to erythromycin, azithromycin, and 25% to clarithromycin. All isolates were susceptible to linezolid, vancomycin, daptomycin and trimethoprim/sulfamethoxazole, and over 97% to rifampicin. The ermA gene was amplified in 88% of HA-MRSA and 17% of CA-MRSA isolates (p < 0.001). The ermC gene was detected in 6% of HA-SARM and none of CA-SARM isolates, whereas the msrA gene was only amplified in 22% of CA-MRSA (p < 0.005). Our results demonstrate the prevalence of the cMLSB resistance phenotype in all HA-MRSA isolates in Chile, with the ermA being the predominant gene identified among these isolates.
dc.description.versionVersión publicada
dc.format.extent8 p.
dc.identifier.citationAntibiotics 2022, 11, 1000.
dc.identifier.doihttps://doi.org/10.3390/antibiotics11081000
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7664
dc.language.isoen
dc.subjectMRSA
dc.subjectMLSB phenotype
dc.subjectAntibiotic-resistant
dc.titlePhenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceAntibiotics
dspace.entity.typePublication

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