Publication: An international, interlaboratory ring trial confirms the feasibility of an extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples
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Abstract
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). "Extraction-less"...
Description
Pettit, Syril; Botten, Jason; Jerome, Keith
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Citation
Mills MG, Bruce E, Huang ML, Crothers JW, Hyrien O, Oura CAL, Blake L, Brown Jordan A, Hester S, Wehmas L, Mari B, Barby P, Lacoux C, Fassy J, Vial P, Vial C, Martinez JRW, Oladipo OO, Inuwa B, Shittu I, Meseko CA, Chammas R, Santos CF, Dionísio TJ, Garbieri TF, Parisi VA, Mendes-Correa MC, de Paula AV, Romano CM, Góes LGB, Minoprio P, Campos AC, Cunha MP, Vilela APP, Nyirenda T, Mkakosya RS, Muula AS, Dumm RE, Harris RM, Mitchell CA, Pettit S, Botten J, Jerome KR. An international, interlaboratory ring trial confirms the feasibility of an extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples. PLoS One. 2022 Jan 13;17(1):e0261853. doi: 10.1371/journal.pone.0261853
Keywords
COVID-19 / diagnosis , COVID-19 / virology , COVID-19 Testing / methods , Feasibility Studies , Humans , Nasopharynx / virology , Pandemics / prevention & control , RNA, Viral / genetics , Real-Time Polymerase Chain Reaction / methods , Reverse Transcription / genetics , SARS-CoV-2 / genetics , Sensitivity and Specificity , Serologic Tests / methods , Specimen Handling / methods
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