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Activation of Intra-nodose Ganglion P2X7 Receptors Elicit Increases in Neuronal Activity

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dc.contributor.authorAlcayaga, Julio
dc.contributor.authorVera, Jorge
dc.contributor.authorReyna, Mauricio
dc.contributor.authorCovarrubias, Alejandra
dc.contributor.authorCoddou, Claudio
dc.contributor.authorDíaz, Esteban
dc.contributor.authorDel Rio, Rodrigo
dc.contributor.authorRetamal, Mauricio A.
dc.date.accessioned2024-01-10T13:32:22Z
dc.date.available2024-01-10T13:32:22Z
dc.date.issued2023
dc.description.abstractVagus nerve innervates several organs including the heart, stomach, and pancreas among others. Somas of sensory neurons that project through the vagal nerve are located in the nodose ganglion. The presence of purinergic receptors has been reported in neurons and satellite glial cells in several sensory ganglia. In the nodose ganglion, calcium depletion-induced increases in neuron activity can be partly reversed by P2X7 blockers applied directly into the ganglion. The later suggest a possible role of P2X7 receptors in the modulation of neuronal activity within this sensory ganglion. We aimed to characterize the response to P2X7 activation in nodose ganglion neurons under physiological conditions. Using an ex vivo preparation for electrophysiological recordings of the neural discharges of nodose ganglion neurons, we found that treatments with ATP induce transient neuronal activity increases. Also, we found a concentration-dependent increase in neural activity in response to Bz-ATP (ED50 = 0.62 mM, a selective P2X7 receptor agonist), with a clear desensitization pattern when applied every ~ 30 s. Electrophysiological recordings from isolated nodose ganglion neurons reveal no differences in the responses to Bz-ATP and ATP. Finally, we showed that the P2X7 receptor was expressed in the rat nodose ganglion, both in neurons and satellite glial cells. Additionally, a P2X7 receptor negative allosteric modulator decreased the duration of Bz-ATP-induced maximal responses without affecting their amplitude. Our results show the presence of functional P2X7 receptors under physiological conditions within the nodose ganglion of the rat, and suggest that ATP modulation of nodose ganglion activity may be in part mediated by the activation of P2X7 receptors.
dc.description.versionVersión Publicada
dc.identifier.citationAlcayaga J, Vera J, Reyna-Jeldes M, Covarrubias AA, Coddou C, Díaz-Jara E, Del Rio R, Retamal MA. Activation of Intra-nodose Ganglion P2X7 Receptors Elicit Increases in Neuronal Activity. Cell Mol Neurobiol. 2023 Aug;43(6):2801-2813. doi: 10.1007/s10571-023-01318-8
dc.identifier.doihttps://doi.org/10.1007/s10571-023-01318-8
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/8321
dc.language.isoen
dc.subjectPurinergic receptor
dc.subjectNodose
dc.subjectP2X
dc.subjectVagal afferences
dc.subjectAdenosine triphosphate
dc.titleActivation of Intra-nodose Ganglion P2X7 Receptors Elicit Increases in Neuronal Activity
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceCellular and molecular neurobiology
dspace.entity.typePublication
relation.isAuthorOfPublication01e4a6fa-e55f-47c5-a1b6-383d5fc6f474
relation.isAuthorOfPublication.latestForDiscovery01e4a6fa-e55f-47c5-a1b6-383d5fc6f474

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