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Whole-genome sequencing reveals changes in genomic diversity and distinctive repertoires of T3SS and T6SS effector candidates in Chilean clinical Campylobacter strains

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dc.contributor.authorKatz, Assaf
dc.contributor.authorPorte, Lorena
dc.contributor.authorWeitzel, Thomas
dc.contributor.authorVarela, Carmen
dc.contributor.authorMuñoz, Cristina
dc.contributor.authorUgalde, Juan
dc.contributor.authorGrim, Christopher
dc.contributor.authorGonzález, Narjol
dc.contributor.authorBlondel, Carlos
dc.contributor.authorBravo, Verónica
dc.date.accessioned2024-05-06T19:22:24Z
dc.date.available2024-05-06T19:22:24Z
dc.date.issued2023
dc.description.abstractCampylobacter is the leading cause of bacterial gastroenteritis worldwide and an emerging and neglected pathogen in South America. This zoonotic pathogen colonizes the gastrointestinal tract of a wide range of mammals and birds, with poultry as the most important reservoir for human infections. Apart from its high morbidity rates, the emergence of resistant strains is of global concern. The aims of this work were to determine genetic diversity, presence of antimicrobial resistance determinants and virulence potential of Campylobacter spp. isolated from patients with acute gastrointestinal disease at 'Clinica Alemana', Santiago de Chile. The study considered the isolation of Campylobacter spp., from stool samples during a 20-month period (January 2020 to September 2021). We sequenced (NextSeq, Illumina) and performed an in-depth analysis of the genome sequences of 88 Campylobacter jejuni and 2 Campylobacter coli strains isolated from clinical samples in Chile. We identified a high genetic diversity among C. jejuni strains and the emergence of prevalent clonal complexes, which were not identified in our previous reports. While ~40% of strains harbored a mutation in the gyrA gene associated with fluoroquinolone resistance, no macrolide-resistance determinants were detected. Interestingly, gene clusters encoding virulence factors such as the T6SS or genes associated with long-term sequelae such as Guillain-Barré syndrome showed lineage-relatedness. In addition, our analysis revealed a high degree of variability regarding the presence of fT3SS and T6SS effector proteins in comparison to type strains 81-176, F38011, and NCTC 11168 and 488. Our study provides important insights into the molecular epidemiology of this emerging foodborne pathogen. In addition, the differences observed regarding the repertoire of fT3SS and T6SS effector proteins could have an impact on the pathogenic potential and transmissibility of these Latin American isolates, posing another challenge in characterizing the infection dynamics of this emergent and neglected bacterial pathogen.
dc.description.versionPublicada
dc.identifier.citationKatz A, Porte L, Weitzel T, Varela C, Muñoz-Rehbein C, Ugalde JA, Grim C, González-Escalona N, Blondel CJ, Bravo V. Whole-genome sequencing reveals changes in genomic diversity and distinctive repertoires of T3SS and T6SS effector candidates in Chilean clinical Campylobacter strains. Front Cell Infect Microbiol. 2023 Jul 13;13:1208825. doi: 10.3389/fcimb.2023.1208825
dc.identifier.doihttps://doi.org/10.3389/fcimb.2023.1208825
dc.identifier.urihttps://hdl.handle.net/11447/8711
dc.language.isoen
dc.subjectT6SS
dc.subjectAntimicrobial resistance
dc.subjectCampylobacteriosis
dc.subjectClonal complexes
dc.subjectfT3SS
dc.subjectMolecular epidemiology
dc.subjectSequence analysis
dc.titleWhole-genome sequencing reveals changes in genomic diversity and distinctive repertoires of T3SS and T6SS effector candidates in Chilean clinical Campylobacter strains
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceFrontiers in cellular and infection microbiology
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscoveryc641ef0c-51cb-4d25-8e9f-c8139bc6a4ad

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