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HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells

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dc.contributor.authorFarías, Mónica
dc.contributor.authorCancino, Felipe
dc.contributor.authorNavarro, Areli
dc.contributor.authorDuarte, Luisa
dc.contributor.authorSoto, Abel
dc.contributor.authorTognarelli, Eduardo
dc.contributor.authorRamm, Maximiliano
dc.contributor.authorAlarcón, Bárbara
dc.contributor.authorCordero, José
dc.contributor.authorSan Martín, Sergio
dc.contributor.authorAgurto, Cristian
dc.contributor.authorRetamal, Angello
dc.contributor.authorRiedel, Claudia
dc.contributor.authorBarrera, Nelson
dc.contributor.authorBustamante, Luis
dc.contributor.authorBueno, Susan
dc.contributor.authorKalergis, Alexis
dc.contributor.authorGonzález, Pablo
dc.date.accessioned2026-08-18T21:44:35Z
dc.date.available2026-08-18T21:44:35Z
dc.date.issued2025
dc.description.abstractHerpes simplex virus type 1 (HSV-1) significantly impairs dendritic cell (DC) function, ultimately eliciting the death of these cells. Here, we sought to assess whether HSV-1 modulates lipid metabolism in mouse DCs as a mechanism of immune evasion. For this, we performed RT-qPCR gene arrays with ingenuity pathway analysis (IPA), RNA sequencing (RNA-seq) and gene set enrichment analysis (GSEA), confocal microscopy, transmission electron microscopy, ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) analysis, pharmacological inhibition of eight lipid-metabolism-related enzymes in HSV-1-infected DCs, co-cultures between virus-specific transgenic CD4+ and CD8+ T cells and HSV-1-infected DCs, and in vivo assays with mice. We found that HSV-1 significantly alters lipid metabolism in DCs and induces lipid droplet (LD) accumulation in these cells. Pharmacological inhibition of two particular lipid metabolism enzymes was found to partially restore DC function. Overall, these results suggest that lipid metabolism plays an important role in the impairment of DC function by HSV-1.
dc.description.versionVersión Publicada
dc.identifier.citationFarías MA, Cancino FA, Navarro AJ, Duarte LF, Soto AA, Tognarelli EI, Ramm MJ, Alarcón-Zapata BN, Cordero J, San Martín S, Agurto-Muñoz C, Retamal-Díaz A, Riedel CA, Barrera NP, Bustamante L, Bueno SM, Kalergis AM, González PA. HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells. iScience. 2025 Apr 15;28(5):112441. doi: 10.1016/j.isci.2025
dc.identifier.doihttps://doi.org/10.1016/j.isci.2025.112441
dc.identifier.urihttps://hdl.handle.net/11447/11019
dc.language.isoen
dc.subjectBiochemistry
dc.subjectBiological sciences
dc.subjectCell biology
dc.subjectImmunology
dc.subjectMicrobiology
dc.subjectNatural
dc.titleHSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceiScience
dspace.entity.typePublication

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