Publication:
Ceftazidime/avibactam resistance is associated with PER-3-producing ST309 lineage in Chilean clinical isolates of non-carbapenemase producing Pseudomonas aeruginosa

Simple item page
dc.contributor.authorSoto, Katherine
dc.contributor.authorAlcalde, Manuel
dc.contributor.authorUgalde, Juan
dc.contributor.authorOlivares, Jorge
dc.contributor.authorQuiroz, Valeria
dc.contributor.authorBrito, Bárbara
dc.contributor.authorRivas Jiménez, Lina María
dc.contributor.authorMunita, Jose M.
dc.contributor.authorGarcía, Patricia
dc.contributor.authorWozniak, Aniela
dc.date.accessioned2025-01-16T13:39:23Z
dc.date.available2025-01-16T13:39:23Z
dc.date.issued2024
dc.description.abstractIntroduction: Ceftazidime/avibactam (CZA) is indicated against multidrug-resistant Pseudomonas aeruginosa, particularly those that are carbapenem resistant. CZA resistance in P. aeruginosa producing PER, a class A extended-spectrum β-lactamase, has been well documented in vitro. However, data regarding clinical isolates are scarce. Our aim was to analyze the contribution of PER to CZA resistance in non-carbapenemase-producing P. aeruginosa clinical isolates that were ceftazidime and/or carbapenem non-susceptible. Methods: Antimicrobial susceptibility was determined through agar dilution and broth microdilution, while bla PER gene was screened through PCR. All PER-positive isolates and five PER-negative isolates were analyzed through Whole Genome Sequencing. The mutational resistome associated to CZA resistance was determined through sequence analysis of genes coding for PBPs 1b, 3 and 4, MexAB-OprM regulators MexZ, MexR, NalC and NalD, AmpC regulators AmpD and AmpR, and OprD porin. Loss of bla PER-3 gene was induced in a PER-positive isolate by successive passages at 43°C without antibiotics. Results: Twenty-six of 287 isolates studied (9.1%) were CZA-resistant. Thirteen of 26 CZA-resistant isolates (50%) carried bla PER. One isolate carried bla PER but was CZA-susceptible. PER-producing isolates had significantly higher MICs for CZA, amikacin, gentamicin, ceftazidime, meropenem and ciprofloxacin than non-PER-producing isolates. All PER-producing isolates were ST309 and their bla PER-3 gene was associated to ISCR1, an insertion sequence known to mobilize adjacent DNA. PER-negative isolates were classified as ST41, ST235 (two isolates), ST395 and ST253. PER-negative isolates carried genes for narrow-spectrum β-lactamases and the mutational resistome showed that all isolates had one major alteration in at least one of the genes analyzed. Loss of bla PER-3 gene restored susceptibility to CZA, ceftolozane/tazobactam and other β-lactamsin the in vitro evolved isolate. Discussion: PER-3-producing ST309 P. aeruginosa is a successful multidrug-resistant clone with blaPER-3 gene implicated in resistance to CZA and other β-lactams.
dc.description.versionVersión Publicada
dc.identifier.citationSoto KD, Alcalde-Rico M, Ugalde JA, Olivares-Pacheco J, Quiroz V, Brito B, Rivas LM, Munita JM, García PC, Wozniak A. Ceftazidime/avibactam resistance is associated with PER-3-producing ST309 lineage in Chilean clinical isolates of non-carbapenemase producing Pseudomonas aeruginosa. Front Cell Infect Microbiol. 2024 Jun 5;14:1410834. doi: 10.3389/fcimb.2024.1410834
dc.identifier.doihttps://doi.org/10.3389/fcimb.2024.1410834
dc.identifier.urihttps://hdl.handle.net/11447/9660
dc.language.isoen
dc.subjectPER-3 extended-spectrum β-lactamase
dc.subjectPseudomonas aeruginosa
dc.subjectBlaPER-3 gene
dc.subjectCeftazidime/avibactam resistance
dc.subjectMutations Conferring CZA resistance
dc.titleCeftazidime/avibactam resistance is associated with PER-3-producing ST309 lineage in Chilean clinical isolates of non-carbapenemase producing Pseudomonas aeruginosa
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceFrontiers in cellular and infection microbiology
dspace.entity.typePublication
relation.isAuthorOfPublication2bf16b44-04d3-45fe-8dd0-dafa6322ca94
relation.isAuthorOfPublicationec52b0bf-d0bc-4844-9531-eca4a65f2b8e
relation.isAuthorOfPublication.latestForDiscovery2bf16b44-04d3-45fe-8dd0-dafa6322ca94

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ceftazidime-avibactam resistance is associated with PER-3-producing ST309 lineage in Chilean.pdf
Size:
1.85 MB
Format:
Adobe Portable Document Format
Description:
Texto Completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
347 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services