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Effects of a High-Fat Diet and Docosahexaenoic Acid during Pregnancy on Fatty Acid Composition in the Fetal Livers of Mice

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dc.contributor.authorÁlvarez, Daniela
dc.contributor.authorOrtiz, Macarena
dc.contributor.authorValdebenito, Gabriel
dc.contributor.authorCrisosto, Nicolás
dc.contributor.authorEchiburú, Bárbara
dc.contributor.authorValenzuela, Rodrigo
dc.contributor.authorEspinoza, Alejandra
dc.contributor.authorMaliqueo, Manuel
dc.date.accessioned2024-06-05T19:55:03Z
dc.date.available2024-06-05T19:55:03Z
dc.date.issued2023
dc.description.abstractA high-fat diet (HFD) during pregnancy promotes fat accumulation and reduces docosahexaenoic acid (DHA) levels in the liver of the offspring at postnatal ages, which can depend on fetal sex. However, the prenatal mechanisms behind these associations are still unclear. Thus, we analyzed if an HFD alters DHA content and the expression of molecules related to fatty acid (FA) metabolism in the fetal liver. Female C57BL/6 mice were fed a control diet or HFD for 4–6 weeks before pregnancy until the gestational day (GD) 17.5. A subgroup of each diet received DHA (100 mg/Kg) orally from GD 6.5 until 16.5. On GD 17.5, maternal livers, placentas, and livers from male and female fetuses were collected for FA profiling with gas-chromatography and gene expression of molecules related to FA metabolism using qPCR. PPAR-α protein expression was evaluated using Western blot. The gene expression of placental FA transporters was also assessed. An HFD increased eicosapentaenoic acid (EPA) and decreased DHA levels and protein expression of PPAR-α in the fetal livers of both sexes. DHA increased the gene expression of Ppara, Cpt1, and Acsl1 in the livers of female fetuses. Therefore, an HFD reduces DHA levels and PPAR-α, a master regulator of gene expression, in the fetal liver. In turn, the livers of female fetuses seem to be more sensitive to DHA action.
dc.description.versionVersión publicada
dc.format.extent15 p.
dc.identifier.citationÁlvarez, D.; Ortiz, M.; Valdebenito, G.; Crisosto, N.; Echiburú, B.; Valenzuela, R.; Espinosa, A.; Maliqueo, M. Effects of a High-Fat Diet and Docosahexaenoic Acid during Pregnancy on Fatty Acid Composition in the Fetal Livers of Mice. Nutrients 2023, 15, 4696. https://doi.org/10.3390/ nu15214696
dc.identifier.doihttps://doi.org/10.3390/nu15214696
dc.identifier.urihttps://hdl.handle.net/11447/9050
dc.language.isoen
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectPregnancy
dc.subjectHigh-fat diet
dc.subjectDocosahexaenoic acid
dc.subjectMaternal liver
dc.subjectPlacenta
dc.subjectFetal liver
dc.titleEffects of a High-Fat Diet and Docosahexaenoic Acid during Pregnancy on Fatty Acid Composition in the Fetal Livers of Mice
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceNutrients
dspace.entity.typePublication

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