Publication:
Skeletal Muscle Atrophy Induced by Diabetes Is Mediated by Non-Selective Channels and Prevented by Boldine

Simple item page
dc.contributor.authorCea, Luis A.
dc.contributor.authorVásquez, Walter
dc.contributor.authorHernández-Salinas, Romina
dc.contributor.authorVielma Z., Alejandra
dc.contributor.authorCastillo-Ruiz, Mario
dc.contributor.authorVelarde, Victoria
dc.contributor.authorSalgado, Magdiel
dc.contributor.authorSáez, Juan C.
dc.date.accessioned2024-05-29T21:11:44Z
dc.date.available2024-05-29T21:11:44Z
dc.date.issued2023
dc.description.abstractIndividuals with diabetes mellitus present a skeletal muscle myopathy characterized by atrophy. However, the mechanism underlying this muscular alteration remains elusive, which makes it difficult to design a rational treatment that could avoid the negative consequences in muscles due to diabetes. In the present work, the atrophy of skeletal myofibers from streptozotocin-induced diabetic rats was prevented with boldine, suggesting that non-selective channels inhibited by this alkaloid are involved in this process, as has previously shown for other muscular pathologies. Accordingly, we found a relevant increase in sarcolemma permeability of skeletal myofibers of diabetic animals in vivo and in vitro due to de novo expression of functional connexin hemichannels (Cx HCs) containing connexins (Cxs) 39, 43, and 45. These cells also expressed P2X7 receptors, and their inhibition in vitro drastically reduced sarcolemma permeability, suggesting their participation in the activation of Cx HCs. Notably, sarcolemma permeability of skeletal myofibers was prevented by boldine treatment that blocks Cx43 and Cx45 HCs, and now we demonstrated that it also blocks P2X7 receptors. In addition, the skeletal muscle alterations described above were not observed in diabetic mice with myofibers deficient in Cx43/Cx45 expression. Moreover, murine myofibers cultured for 24 h in high glucose presented a drastic increase in sarcolemma permeability and levels of NLRP3, a molecular member of the inflammasome, a response that was also prevented by boldine, suggesting that, in addition to the systemic inflammatory response found in diabetes, high glucose can promote the expression of functional Cx HCs and activation of the inflammasome in skeletal myofibers. Therefore, Cx43 and Cx45 HCs play a critical role in myofiber degeneration, and boldine could be considered a potential therapeutic agent to treat muscular complications due to diabetes.
dc.description.versionVersión publicada
dc.format.extent14 p.
dc.identifier.citationCea, L.A.; Vásquez, W.; Hernández-Salinas, R.; Vielma, A.Z.; Castillo-Ruiz, M.; Velarde, V.; Salgado, M.; Sáez, J.C. Skeletal Muscle Atrophy Induced by Diabetes Is Mediated by Non-Selective Channels and Prevented by Boldine. Biomolecules 2023, 13, 708. https:// doi.org/10.3390/biom13040708
dc.identifier.doihttps://doi.org/10.3390/biom13040708
dc.identifier.urihttps://hdl.handle.net/11447/8943
dc.language.isoen
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectCalcium atrophy
dc.subjectConnexins
dc.subjectHemichannel blocker
dc.subjectSarcolemma permeability
dc.titleSkeletal Muscle Atrophy Induced by Diabetes Is Mediated by Non-Selective Channels and Prevented by Boldine
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceBiomolecules
dspace.entity.typePublication

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
biomolecules-13-00708.pdf
Size:
2.59 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
347 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services