Publication:
KFERQ-selective protein autophagy in Caenorhabditis elegans depends on LMP-1

Simple item page
dc.contributor.authorGallardo, María
dc.contributor.authorMinniti, Alicia
dc.contributor.authorHormazabal, Juan
dc.contributor.authorNúñez, Gonzalo
dc.contributor.authorLagos, Carlos
dc.contributor.authorPerez-Acle, Tomás
dc.contributor.authorAldunate, Rebeca
dc.contributor.authorAlfaro, Ivan
dc.date.accessioned2026-11-04T14:12:21Z
dc.date.available2026-11-04T14:12:21Z
dc.date.issued2025
dc.description.abstractMammalian cells exhibit three autophagy mechanisms: macroautophagy, microautophagy (MIA), and chaperone-mediated autophagy (CMA), each employing unique mechanisms for transporting cellular material to the lysosome for degradation. MIA involves the engulfment of proteins via lysosomes/late endosomes through membrane invagination, while CMA directly imports cytosolic proteins into lysosomes, selectively targeting those harboring the KFERQ pentapeptide motif, helped by the chaperone HSC70. Despite the identification of several genetic markers of these pathways, our understanding of the underlying mechanisms, particularly in MIA and CMA, remains limited. To study CMA in vivo we designed a photoactivatable CMA reporter consisting of a plasmid encoding the KFERQ consensus signal for CMA targeting. We generated transgenic C. elegans strains with diverse genetic backgrounds to analyze the role of known molecular components of CMA in mammals. Additionally, we conducted an in-silico analysis of the structural interaction between C. elegans LMP-1 or LMP-2 proteins with the HSP-1 chaperone. Results: Our study shows a significant alteration in the distribution pattern of the KFERQ reporter in muscle cells upon induction of selective autophagy (CMA or MIA). We found that the reporter localized into lysosomes only during starvation, which abrogated in the absence of LMP-1. This study validates CMA in C. elegans and provides the development of a new tool for understanding selective autophagy mechanisms and their potential implications in various organisms.
dc.description.versionVersión Publicada
dc.identifier.citationGallardo-Campos, M., Minniti, A. N., Hormazabal, J., Núñez, G., Lagos, C. F., Perez-Acle, T., Aldunate, R., & Alfaro, I. E. (2025). KFERQ-selective protein autophagy in Caenorhabditis elegans depends on LMP-1. PloS one, 20(9), e0330339. https://doi.org/10.1371/journal.pone.0330339
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0330339
dc.identifier.urihttps://hdl.handle.net/11447/11316
dc.language.isoen
dc.subjectCaenorhabditis elegans Proteins* / chemistry
dc.subjectCaenorhabditis elegans Proteins* / genetics
dc.subjectCaenorhabditis elegans Proteins* / metabolism
dc.subjectChaperone-Mediated Autophagy
dc.subjectGenetically Modified
dc.subjectHSC70 Heat-Shock Proteins / metabolism
dc.titleKFERQ-selective protein autophagy in Caenorhabditis elegans depends on LMP-1
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourcePloS one
dspace.entity.typePublication
relation.isAuthorOfPublicationd0fb8162-7e81-4714-92e6-55df118f11c3
relation.isAuthorOfPublication57eddc30-b34b-4af7-869d-ed5152bfa3d6
relation.isAuthorOfPublication.latestForDiscoveryd0fb8162-7e81-4714-92e6-55df118f11c3

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
KFERQ-selective protein autophagy in Caenorhabditis elegans depends on LMP-1.pdf
Size:
2.79 MB
Format:
Adobe Portable Document Format
Description:
Texto Completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
347 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services