Publication:
Reduced microbial diversity of the nasopharyngeal microbiome in household contacts with latent tuberculosis infection

Simple item page
dc.contributor.authorRuiz‑Tagle, Cinthya
dc.contributor.authorUgalde, Juan
dc.contributor.authorNaves, Rodrigo
dc.contributor.authorAraos Bralic, Rafael Ignacio
dc.contributor.authorGarcía, Patricia
dc.contributor.authorBalcells, María
dc.date.accessioned2024-06-03T17:54:33Z
dc.date.available2024-06-03T17:54:33Z
dc.date.issued2023
dc.description.abstractThe upper respiratory tract is an obliged pathway for respiratory pathogens and a healthy microbiota may support the host's mucosal immunity preventing infection. We analyzed the nasopharyngeal microbiome in tuberculosis household contacts (HHCs) and its association with latent tuberculosis infection (TBI). A prospective cohort of HHCs was established and latent TBI status was assessed by serial interferon-γ release assay (IGRA). Nasopharyngeal swabs collected at baseline were processed for 16S rRNA gene sequencing. The 82 participants included in the analysis were classified as: (a) non-TBI [IGRA negative at baseline and follow-up, no active TB (n = 31)], (b) pre-TBI [IGRA negative at baseline but converted to IGRA positive or developed active TB at follow-up (n = 16)], and (c) TBI [IGRA positive at enrollment (n = 35)]. Predominant phyla were Actinobacteriota, Proteobacteria, Firmicutes and Bacteroidota. TBI group had a lower alpha diversity compared to non-TBI (padj = 0.04) and pre-TBI (padj = 0.04). Only TBI and non-TBI had beta diversity differences (padj = 0.035). Core microbiomes' had unique genera, and genus showed differential abundance among groups. HHCs with established latent TBI showed reduced nasopharyngeal microbial diversity with distinctive taxonomical composition. Whether a pre-existing microbiome feature favors, are a consequence, or protects against Mycobacterium tuberculosis needs further investigation.
dc.description.versionPublicada
dc.identifier.citationRuiz-Tagle C, Ugalde JA, Naves R, Araos R, García P, Balcells ME. Reduced microbial diversity of the nasopharyngeal microbiome in household contacts with latent tuberculosis infection. Sci Rep. 2023 May 5;13(1):7301. doi: 10.1038/s41598-023-34052-8.
dc.identifier.doihttps://doi.org/10.1038/s41598-023-34052-8
dc.identifier.urihttps://hdl.handle.net/11447/8997
dc.language.isoen
dc.subjectInterferon-gamma Release Tests
dc.subjectMycobacterium tuberculosis/ genetics
dc.subjectLatent Tuberculosis / microbiology
dc.subjectProspective Studies
dc.subject16S / genetics
dc.subjectRNA Ribosomal 16S
dc.subjectTuberculosis
dc.titleReduced microbial diversity of the nasopharyngeal microbiome in household contacts with latent tuberculosis infection
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceScientific reports
dspace.entity.typePublication
relation.isAuthorOfPublication69e40056-7a55-41b3-8a65-05aace0e07e3
relation.isAuthorOfPublication.latestForDiscovery69e40056-7a55-41b3-8a65-05aace0e07e3

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Reduced microbial diversity of the nasopharyngeal microbiome in household contacts with latent tuberculosis infection.pdf
Size:
2.3 MB
Format:
Adobe Portable Document Format
Description:
Texto Completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
347 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services