Publication:
Concordance of Helicobacter pylori Detection Methods in Symptomatic Children and Adolescents

Simple item page
dc.contributor.authorCabrera, Camila
dc.contributor.authorCampusano, Yanira
dc.contributor.authorTorres, Joaquín
dc.contributor.authorIvulic, Dinka
dc.contributor.authorGalvez, Valeria
dc.contributor.authorTapia, Diego
dc.contributor.authorRodríguez, Vicente
dc.contributor.authorLagomarcino, Anne
dc.contributor.authorGallardo, Alejandra
dc.contributor.authorAlliende, Francisco
dc.contributor.authorToledo, Marcela
dc.contributor.authorRomán, Gabriela
dc.contributor.authorJaime, Francisca
dc.contributor.authorGonzález, Mónica
dc.contributor.authorMarchant, Pamela
dc.contributor.authorRojas, Marianela
dc.contributor.authorJuanet, Juan Ignacio
dc.contributor.authorVillanueva, Mónica
dc.contributor.authorOssa, Juan Cristóbal
dc.contributor.authorDel Canto, Felipe
dc.contributor.authorViver, Tomeu
dc.contributor.authorO’Ryan, Miguel
dc.contributor.authorLucero, Yalda
dc.date.accessioned2026-08-10T20:20:28Z
dc.date.available2026-08-10T20:20:28Z
dc.date.issued2025
dc.description.abstractBackground: Helicobacter pylori is the most prevalent chronic bacterial infection globally, acquired mostly during childhood. It is associated with chronic gastritis, peptic ulcer disease, and gastric cancer. Due to challenges in culturing H. pylori, diagnostic reference standards often rely on combining ≥2 non-culture, biopsy-based methods. Histology with Giemsa staining is widely used in clinical settings due to its low cost and reliable performance. Methods: This study evaluated the concordance between histology with Giemsa staining as the reference standard and other diagnostic methods, including the rapid urease test (RUT), ureA RT-PCR, 16S sequencing, and anti-H. pylori serum IgG. Positive percent of agreement (PPA), negative percent of agreement (NPA) and concordance kappa index were calculated. Results: A total of 120 patients (41 positive and 79 negative by Giemsa staining) were analyzed. Among the methods tested, RT-PCR for ureA showed the best performance (PPA = 94.7%, NPA = 98.6%, kappa = 0.939), while RUT underperformed compared with expectations (PPA = 65.9%, NPA = 97.5%, kappa = 0.681). Serology had the lowest performance (PPA = 53.7%, NPA = 96.1%, kappa = 0.548). Conclusions: The combination of histology with Giemsa staining and ureA RT-PCR achieved the highest detection rate and strongest agreement.
dc.description.versionVersión publicada
dc.format.extent13 p.
dc.identifier.citationCabrera, C.; Campusano, Y.; Torres, J.; Ivulic, D.; Galvez, V.; Tapia, D.; Rodríguez, V.; Lagomarcino, A.; Gallardo, A.; Alliende, F.; et al. Concordance of Helicobacter pylori Detection Methods in Symptomatic Children and Adolescents. Microorganisms 2025, 13, 583. https://doi.org/10.3390/ microorganisms13030583
dc.identifier.doihttps://doi.org/10.3390/ microorganisms13030583
dc.identifier.urihttps://hdl.handle.net/11447/10988
dc.language.isoen
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectHelicobacter pylori
dc.subjectChildren
dc.subjectAdolescents
dc.subjectHistology
dc.subjectRT-PCR
dc.subjectRapid urease test
dc.subjectSerology
dc.subjectGastric microbiome
dc.titleConcordance of Helicobacter pylori Detection Methods in Symptomatic Children and Adolescents
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceMicroorganisms
dspace.entity.typePublication

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
microorganisms-13-00583-v3.pdf
Size:
1.76 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
347 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services