Publication: Novel sponge formulation of mesenchymal stem cell secretome and hyaluronic acid: a safe and effective topical therapy for Psoriasis vulgaris
| dc.contributor.author | Elgueta, Estefanía | |
| dc.contributor.author | Prieto, Catalina P. | |
| dc.contributor.author | Hartmann, Dan | |
| dc.contributor.author | Carrillo, Daniela | |
| dc.contributor.author | Edwards, Natalie | |
| dc.contributor.author | Martínez, Constanza | |
| dc.contributor.author | Pérez, Dan | |
| dc.contributor.author | Casas, Bárbara | |
| dc.contributor.author | Vergara, Luis | |
| dc.contributor.author | Valenzuela, Fernando | |
| dc.contributor.author | Catalán, Verónica | |
| dc.contributor.author | Lattus, José | |
| dc.contributor.author | Oyarzun-Ampuero, Felipe | |
| dc.contributor.author | Palma, Verónica | |
| dc.date.accessioned | 2026-07-30T21:19:33Z | |
| dc.date.available | 2026-07-30T21:19:33Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Background Psoriasis vulgaris is the most common form of psoriasis, yet current treatments often lead to significant side effects, resulting in a high rate of therapy desertion. Here, we explored a novel therapeutic approach using the secretome from Wharton Jelly-derived mesenchymal stem cells, biologically stabilized and enhanced with hyaluronic acid (HA), its presentation is an easy-to-apply topical sponge. This formulation had previously demonstrated efficacy in vitro and in experimental psoriasis mouse models. Methods In vitro characterization studies included dynamic light scattering, nanoparticle tracking analysis, optical/electronic microscopy, microbiological experiments, and angiogenic capacity (HUVEC cells). In vivo studies included angiogenic capacity in chicken embryo chorioallantoic membrane (CAM), safety (hypersensitive and healthy volunteers), and efficacy (double-blinded and randomized patients). Results We demonstrated the presence of spherical exosomes (164 ± 87 nm, PDI of 0.38, and 1.5 × 10⁷ particles/mL) within the selected secretomes, which exhibited significant proangiogenic activity in HUVEC cells and in a CAM assay. The secretome-containing sponges displayed distinct physicochemical properties, such as the absence of nitrogen and reduced carbon and oxygen content, resulting in a more cross-linked material with thinner fibers. These characteristics extended the dispersion time in aqueous media. Microbiological testing confirmed sterility in the packed, ready-to-use secretome-HA sponges after 3 months of storage. To assess safety, we selected doses (based on total protein content) that were applied to three patients with atopic dermatitis (42 μg of protein, patch test, 5 days) and four healthy volunteers (210 μg, 15 days) with no observed adverse topical or systemic effects. In a 30-day. | |
| dc.description.version | Versión publicada | |
| dc.format.extent | 15 p. | |
| dc.identifier.citation | Elgueta E, Prieto CP, Hartmann D, Carrillo D, Edwards N, Martínez C, Pérez D, Casas B, Vergara L, Valenzuela F, Catalán V, Lattus J, Oyarzun-Ampuero F, Palma V. Novel sponge formulation of mesenchymal stem cell secretome and hyaluronic acid: a safe and effective topical therapy for Psoriasis vulgaris. Stem Cell Res Ther. 2025 Jul 6;16(1):348. doi: 10.1186/s13287-025-04415-1. | |
| dc.identifier.doi | https://doi.org/10.1186/s13287-025-04415-1 | |
| dc.identifier.uri | https://hdl.handle.net/11447/10941 | |
| dc.language.iso | en | |
| dc.title | Novel sponge formulation of mesenchymal stem cell secretome and hyaluronic acid: a safe and effective topical therapy for Psoriasis vulgaris | |
| dc.type | Article | |
| dcterms.accessRights | Acceso abierto | |
| dcterms.source | Stem Cell Research & Therapy | |
| dspace.entity.type | Publication |