Antifungal activity of “HO21-F”, a formulation based on Olea europaea plant extract, in honey bees infected with Nosema ceranae

Duguet, Jos!e; Zuñiga, Fabián; Martínez, Jessica

Publication:
Antifungal activity of “HO21-F”, a formulation based on Olea europaea plant extract, in honey bees infected with Nosema ceranae

dc.contributor.author	Duguet, Jos!e
dc.contributor.author	Zuñiga, Fabián
dc.contributor.author	Martínez, Jessica
dc.date.accessioned	2023-04-26T13:58:52Z
dc.date.available	2023-04-26T13:58:52Z
dc.date.issued	2022
dc.description.abstract	Nosema ceranae is a microsporidium parasite that silently affects honey bees, causing a disease called nosemosis. This parasite produces resistant spores and germinates in the midgut of honey bees, extrudes a polar tubule that injects an infective sporoplasm in the host cell epithelium, proliferates, and produces intestinal disorders that shorten honey bee lifespan. The rapid extension of this disease has been reported to be widespread among adultbees, and treatments are less effective and counterproductive weakening colonies. This work aimed to evaluatethe antifungal activity of a prototype formulation based on a non-toxic plant extract (HO21-F) against N􀀤 ceranae. In laboratory, honey bees were infected artificially, kept in cages for 17 days and samples were taken at 7 and 14 days post infection (dpi). At the same time, in field conditions we evaluated the therapeutic effect of HO21-F for 28 days in naturally infected colonies. The effectiveness of the treatment has been demonstrated by a reduction of 83.6 % of the infection levels observed in laboratory conditions at concentrations of 0.5 and 1 g/L without affecting the survival rate. Besides, in-field conditions we reported a reduction of 88 % of the infection level at a concentration of 2.5 g/L, obtaining better antifungal effectiveness in comparison to other commercially available treatments. As a result, we observed that the use of HO21-F led to an increase in population size and honey production, both parameters associated with colony strength. The reported antifungal activity of HO21-F against N. ceranae, with a significant control of spore proliferation in worker bees, suggests the promising commercial application use of this product against nosemosis, and it will encourage new research studies to understand the mechanism of action, whether related to the spore-inhibition effect and/or a stimulating effect in natural response of colonies to counteract the disease.
dc.description.version	Versión Publicada
dc.identifier.citation	José Duguet, Fabian Zuñiga, Jessica Martínez."Antifungal activity of “HO21-F”, a formulation based on Olea europaea plant extract, in honey bees infected with Nosema ceranae”. Journal of Invertebrate Pathology 193 (2022) 107801. Available online 19 July 2022. https://doi.org/10.1016/j.jip.2022.107801
dc.identifier.doi	https://doi.org/10.1016/j.jip.2022.107801
dc.identifier.uri	https://repositorio.udd.cl/handle/11447/7390
dc.language.iso	en
dc.subject	Nosema ceranae
dc.subject	Honey bee
dc.subject	Infection level
dc.subject	Antifungal activity
dc.subject	Plant extract
dc.subject	Olea europaea
dc.title	Antifungal activity of “HO21-F”, a formulation based on Olea europaea plant extract, in honey bees infected with Nosema ceranae
dc.type	Article
dcterms.accessRights	Embargo
dcterms.source	Journal of Invertebrate Pathology
dspace.entity.type	Publication