Browsing by Author "Vidal, Rodrigo"

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    Identification and characterization of miRNAs and lncRNAs of coho salmon (Oncorhynchus kisutch) in normal immune organs
    (Elsevier Inc, 2020) Leiva, Francisco; Rojas-Herrera, Marcelo; Reyes, Daniela; Bravo, Scarleth; García, Killen; Moya, Javier; Vidal, Rodrigo
    MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) are two relevant non-coding RNAs (ncRNAs) class. Oncorhynchus kisutch (coho salmon) is an important aquaculture pacific salmon species without report of miRNAs and a very limited register of lncRNAs. To gain knowledge about the interaction and discovery of miRNAs and lncRNAs in coho salmon we used high-throughput sequencing technology to sequence small and transcriptome libraries from three immune organs. A total of 163 mature miRNAs and 4,975 lncRNAs were discovered. The profiles of expression of both ncRNAs indicated that liver and head-kidney share relatively similar expression patterns. We identified 814 and 181 putative target sequences for 1048 lncRNAs and 47 miRNAs, respectively. The results obtained provide new information and enlarge our understanding of the diversities of ncRNAs in coho salmon.
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    Metal concentrations and source identification in Chilean public children's playgrounds
    (2018) Rodríguez-Oroz, Delia; Vidal, Rodrigo; Fernandoy, Francisco; Lambert, Fabrice; Quiero, Felipe
    This study is focused on four Biobio region cities, Concepcion, Talcahuano, Los Ángeles, and Tomé; these cities flourished very close to different industrial activities. We determined a pseudo total concentration of seven heavy metals (Cr, Ni, Cu, Zn, As, Cd, and Pb) in playground soils through inductively coupled plasma-mass spectrometry (ICP-MS). Principal components analysis (PCA) revealed that contamination in soils comes from three principal sources. Firstly, industrial and burning activities; secondly, the use of phytosanitary and chemical products; and thirdly, vehicular traffic emissions. Zn and Cu are the most abundant analyzed elements in all the playground's soils. Concepción reflected the lowest values of pollutants and Talcahuano the highest, reflecting the industrial effects. The average values of the analyzed elements were Cr = 32.90 mg kg-1; Ni = 23.76 mg kg-1; Cu = 31.51 mg kg-1; Zn = 63.69 mg kg-1; As = 19.51 mg kg-1; Cd = 0.50 mg kg-1; and Pb = 17.59 mg kg-1. Anomalously high values of some elements were found Cu = 462.73 mg kg-1, Zn = 364.39 mg kg-1, As = 34.7 mg kg-1 in Talcahuano, Cd = 1.6 mg kg-1 in Tome, and Pb = 55.59 mg kg-1 in Los Ángeles. Nevertheless, according to international guideline values of pollutants (VROM 2000 and ADEC 2010) there is no risk for children in any playground studied but all playgrounds are a potential risk for the environment. It points out the necessity to continue studying and monitoring Chilean urban playground to prevent health problems in the population. Graphical abstract.

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