Browsing by Author "Salas, Francisca"

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    Folate and Vitamin B12 Levels in Chilean Women with PCOS and Their Association with Metabolic Outcomes
    (2024) Carrasco, Matías; Silveira, Taís; Martínez, Paz; Cerpa, Leslie; Calfunao, Susan; Echiburú, Bárbara; Maliqueo, Manuel; Crisosto, Nicolás; Salas, Francisca
    Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects women of reproductive age. Many women with PCOS have been found to have an unbalanced diet and deficiencies in essential nutrients. This study aimed to assess the levels of folate and vitamin B12 (B12) and their relationship with metabolic factors in women with PCOS. Anthropometric, clinical, and genetic analyses were conducted to evaluate markers related to one-carbon metabolism in women with PCOS and in a control group. The PCOS group had a higher BMI and HOMA-IR (1.7 vs. 3.1; p < 0.0001). HDL cholesterol levels were 23% lower and triglyceride levels were 74% higher in women with PCOS. Although there were no significant differences in folate and B12 levels between the PCOS and control groups, over 60% of women with PCOS had low B12 levels (<300 pg/mL) and high homocysteine levels. In addition, the MTHFR A1298C and C677T polymorphisms were not associated with PCOS. Moreover, erythrocyte folate levels were positively correlated with fasting glucose, triglycerides, and free androgen index, and negatively correlated with SHBG and LH levels. These results suggest that B vitamins may be associated with the metabolic phenotype in PCOS. This study emphasizes the potential link between folate, vitamin B12, and metabolic and hormonal outcomes in women with PCOS.
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    Oxidative Stress in Polycystic Ovary Syndrome: Impact of Combined Oral Contraceptives
    (2024) Santander, Nicolás; Figueroa, Esteban; González, Alejandro; Maliqueo, Manuel; Echiburú, Bárbara; Crisosto, Nicolás; Salas, Francisca
    Polycystic Ovary Syndrome (PCOS) is a complex hormonal disorder that is associated with heightened metabolic risks. While oxidative stress (OS) is known to play a role in PCOS, the precise nature of the relationship between PCOS and increased OS remains not entirely understood. Combined oral contraceptives (COCs) are the first-line treatment to regulate menstrual cycles and androgen levels, but their impact on oxidative stress requires further study. We conducted a transcriptomic analysis using RNAseq and assessed the levels of various oxidative stress (OS) markers in serum samples from women with PCOS and controls and whether they were using combined oral contraceptives (COCs), including enzymatic activities, FRAP, and 8-isoprostane (8-iso). A total of 359 genes were differentially expressed in women with PCOS compared to control women. Genes differentially expressed were enriched in functions related to inflammation and, interestingly, oxidative stress response. In controls, 8-iso levels were increased in women using COCs, whereas in women with PCOS, 8-iso levels were reduced in those using oral contraceptives (191.1 ± 97 vs. 26.4 ± 21 pg/mL, p: <0.0001). Correlation analyses showed a trend for a negative correlation between 8-iso and Ferriman score in women with PCOS consuming COCs (r = -0.86, p = 0.06) and a negative correlation between GSH and hyperandrogenism in women with PCOS (r = -0.89, p = 0.01). These results reveal the presence of lipid peroxidation in women with PCOS, which was modified by the use of COCs, providing new insights into the pathophysiology of PCOS in the Chilean population.
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    Proyecto Espacio Humano: museo y jardín. San Carlos de Apoquindo, Chile, 2023.
    (2023) Bahamondes, Eloy; Vásquez, Lucas; Cox, Marcelo; Jullien, François; Zarhi, Sebastián; Romero, Camila; Salas, Francisca; González, Elena
    Para las y los autores de este proyecto, ganador del concurso para albergar la obra del escultor Mario Irarrázabal, un museo puede entenderse como un jardín -definido por sus límites, pero también abierto al cambio-. Para nosotros en ARQ, este proyecto también demuestra cómo los edificios pueden ser herramientas para la recuperación ecológica, donde las lógicas de emplazamiento, circulación y redes de infraestructura se tratan como oportunidades para favorecer a las especies más-que-humanas que ya habitan este lugar.

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