Browsing by Author "Saint Jean, Nicole"
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Item Mesenchymal Stem Cells Derived from Human Inflamed Dental Pulp Exhibit Impaired Immunomodulatory Capacity In Vitro(2020) Inostroza, Carolina; Vega-Letter, Ana María; Brizuela, Claudia; Castrillón, Luis; Saint Jean, Nicole; Mira Duran, Carol; Carrión, FlavioIntroduction:Dental pulp stem cells (DPSC) are very attractive in regenerative medicine. Inthis study, we focused on the characterization of the functional properties of mesenchymalstem cells derived from DPSCs. Currently, it is unknown whether inflammatory conditionspresent in an inflamed dental pulp tissue could alter the immunomodulatory properties ofDPSCs. This study aimed to evaluate the immunomodulatory capacityin vitroof DPSCsderived from healthy and inflamed dental pulp.Methods:DPSCs from 10 healthy andinflamed dental pulps (irreversible pulpitis) were characterized according to the minimal criteriaof the International Society for Cell Therapy, proliferation, differential potential, and colony-forming units. Furthermore, the immunomodulatory capacity of DPSCs was tested on theproliferation of T lymphocytes byflow cytometry and thein vitroenzyme activity of indoleamine2, 3-dioxygenase. Results:There were no significant differences in the DPSC characteristicsand properties such as immunophenotype, tridifferentiation, colony-forming units, and pro-liferation of the DPSCs derived from normal and inflamed pulp tissue. Furthermore, there weresignificant differences in the immunomodulatory capacity of DPSCs obtained from humanhealthy dental pulp and with the diagnosis of irreversible pulpitis. Conclusions:Our resultsshowed that DPSCs isolated from inflamed dental pulp showed typical characteristics ofMSCs and diminished immunosuppressive capacityin vitroin comparison with MSCs derivedfrom healthy dental pulp. Further investigationin vivois needed to clarify the mechanism of thisdiminished immunosuppressive capacity.