Browsing by Author "Lemaitre, Dominique"
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Item A REDCap application that links researchers, animal facility staff and members of the IACUC in animal health monitoring(Sage Journals, 2019) Carvajal, Cristobal; Vallejos, Catalina; Lemaitre, Dominique; Ruiz, Jorge; Guzmán, Camila; Aguilera, Valentina; Baño, Diego; Calligaris, SebastiánLos estudios de investigación que requieren la experimentación con animales son regulados por el Comité Institucional para el Cuidado y Uso de Animales de Laboratorio (CICUAL). Con este fin, el CICUAL debe integrar la información suministrada por los investigadores de cada estudio preclínico y por los veterinarios de Bioterio para poder controlar y aprobar el proceso. Utilizar un sistema en papel para recoger datos del bienestar y la salud de los animales es una práctica común pero que requiere mucho tiempo, propensa a errores de lectura y transcripción, sin nombrar todos los inconvenientes que tiene para veterinarios e investigadores que desean tomar decisiones colaborativas y precisas cuando el bienestar del animal está en peligro. Hemos creado un Sistema Web para gestión de bases de datos que se centra en la salud animal con el potencial de mejorar su bienestar. El sistema de gestión de datos utiliza el software REDCap, que permite la integración de datos para ofrecer una solución para la evaluación del bienestar animal. El programa propuesto incluye indicadores claves del estado de salud general, como el entorno, información física/nutricional y parámetros de comportamiento durante la cría y la experimentación con animales, como componentes importantes del bienestar animal. Asimismo, el sistema facilita la comunicación de esta información entre los investigadores, el personal de Bioterio y el CICUAL. REDCap está disponible para organizaciones sin fines de lucro de lucro y adáptandose a los requerimientos propios de las instituciones interesadas y responsables del cuidado de animales utilizados en los estudios de investigación. REDCap es una herramienta excelente para fomentar buenas prácticas que beneficien la salud de los animales de experimentación.Publication Capture and detection of extracellular vesicles derived from human breast cancer cells using a 3D self-assembled nanostructured SiO2 microfluidic chip(2025) Cabeza, Carolina; Rojas, Felipe; Lobos, Lorena; Lemaitre, Dominique; Villena, Juan; Cordero, María; Hassan, Natalia; Ortiz, RinaBackground: Tumor-derived extracellular vesicles offer a minimally invasive approach to evaluate tumor progression and metastasis. However, detecting biomarkers, such as extracellular vesicles in body fluids during the early stages of disease, remains a significant challenge. Conventional methods like ultracentrifugation-based isolation or Western blot protein quantification are time-consuming, require large sample volumes, and offer low yield and sensitivity. Therefore, the development of new biosensors for the specific and efficient analysis of tumor extracellular vesicles is urgently needed. Methods: Microfluidic devices provide extraordinary benefits for bioanalysis, offering a large surface area for the contact between target molecules and the biosensor, significantly enhancing the specificity, efficiency, and speed. These devices also enable nanoscale and microscale work using reduced sample volumes. In this study, we developed a three-dimensional self-assembled SiO2-based nanostructured microfluidic chip, bioconjugated with specific antibodies targeting exosomal markers for the selective capture of CD63- and CD81-positive extracellular vesicles from breast cancer-derived conditioned cell culture media. Results: The three-dimensional SiO2-based microfluidic chip effectively captured extracellular vesicles expressing CD63 and CD81 antigens from breast cancer cell culture media. This evidence demonstrates the potential of this platform to detect extracellular vesicles as biomarkers for cancer, providing a specific and efficient, non-invasive approach for cancer diagnostics. Conclusions: This study highlights the potential application of three-dimensional SiO2-based microfluidic chips for detecting extracellular vesicles as a non-invasive liquid biopsy tool for breast cancer. The findings show a specific and efficient device as an alternative to conventional biomarker detection techniques.Item Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile(2020) Lemaitre, Dominique; Llavero Hurtado, Maica; Gregorio, Cristian de; Oñate, Maritza; Martínez, Gabriela; Catenaccio, Alejandra; Wishart, Thomas M.; Court, Felipe A.Peripheral nerve injuries result in motor and sensory dysfunction which can be recovered by compensatory or regenerative processes. In situations where axonal regeneration of injured neurons is hampered, compensation by collateral sprouting from uninjured neurons contributes to target reinnervation and functional recovery. Interestingly, this process of collateral sprouting from uninjured neurons has been associated with the activation of growth-associated programs triggered by Wallerian degeneration. Nevertheless, the molecular alterations at the transcriptomic level associated with these compensatory growth mechanisms remain to be fully elucidated. We generated a surgical model of partial sciatic nerve injury in mice to mechanistically study degenerationinduced collateral sprouting from spared fibers in the peripheral nervous system. Using next-generation sequencing and Ingenuity Pathway Analysis, we described the sprouting-associated transcriptome of uninjured sensory neurons and compare it with the activated by regenerating neurons. In vitro approaches were used to functionally assess sprouting gene candidates in the mechanisms of axonal growth. Using a novel animal model, we provide the first description of the sprouting transcriptome observed in uninjured sensory neurons after nerve injury. This collateral sprouting-associated transcriptome differs from that seen in regenerating neurons, suggesting a molecular program distinct from axonal growth. We further demonstrate that genetic upregulation of novel sproutingassociated genes activates a specific growth program in vitro, leading to increased neuronal branching. These results contribute to our understanding of the molecular mechanisms associated with collateral sprouting in vivo. The data provided here will therefore be instrumental in developing therapeutic strategies aimed at promoting functional recovery after injury to the nervous system.