Browsing by Author "Jaime, Francisca"

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    Concordance of Helicobacter pylori Detection Methods in Symptomatic Children and Adolescents
    (2025) Cabrera, Camila; Campusano, Yanira; Torres, Joaquín; Ivulic, Dinka; Galvez, Valeria; Tapia, Diego; Rodríguez, Vicente; Lagomarcino, Anne; Gallardo, Alejandra; Alliende, Francisco; Toledo, Marcela; Román, Gabriela; Jaime, Francisca; González, Mónica; Marchant, Pamela; Rojas, Marianela; Juanet, Juan Ignacio; Villanueva, Mónica; Ossa, Juan Cristóbal; Del Canto, Felipe; Viver, Tomeu; O’Ryan, Miguel; Lucero, Yalda
    Background: Helicobacter pylori is the most prevalent chronic bacterial infection globally, acquired mostly during childhood. It is associated with chronic gastritis, peptic ulcer disease, and gastric cancer. Due to challenges in culturing H. pylori, diagnostic reference standards often rely on combining ≥2 non-culture, biopsy-based methods. Histology with Giemsa staining is widely used in clinical settings due to its low cost and reliable performance. Methods: This study evaluated the concordance between histology with Giemsa staining as the reference standard and other diagnostic methods, including the rapid urease test (RUT), ureA RT-PCR, 16S sequencing, and anti-H. pylori serum IgG. Positive percent of agreement (PPA), negative percent of agreement (NPA) and concordance kappa index were calculated. Results: A total of 120 patients (41 positive and 79 negative by Giemsa staining) were analyzed. Among the methods tested, RT-PCR for ureA showed the best performance (PPA = 94.7%, NPA = 98.6%, kappa = 0.939), while RUT underperformed compared with expectations (PPA = 65.9%, NPA = 97.5%, kappa = 0.681). Serology had the lowest performance (PPA = 53.7%, NPA = 96.1%, kappa = 0.548). Conclusions: The combination of histology with Giemsa staining and ureA RT-PCR achieved the highest detection rate and strongest agreement.
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    Evaluación de presencia y severidad de periodontitis en pacientes chilenos con artritis reumatoide atendidos en el Hospital Padre Hurtado
    (2015) Ibáñez, Sebastián; Ferreira, Cristina; Contreras, Andrés; Valenzuela, Omar; Giadalah, Nicolás; Jara, Vicente; Jaime, Francisca; Jarpa, Elena
    Background: Periodontitis may have a triggering and aggravating role of various medical conditions, including rheumatoid arthritis. Aim: To evaluate the periodontal status in Chilean patients with rheumatoid arthritis (RA), treated in a public hospital. Patients and Methods: A trained professional conducted a periodontal examination in 40 patients with RA aged 23 to 73 years (85% women). When present, the severity of periodontitis and its relationship with gender, smoking, age, corticosteroids dose and AR activity were assessed. AR activity was evaluated using the Disease Activity Score Calculator for Rheumatoid Arthritis (DAS 28). Results: Thirty five of the 40 patients had periodontitis and in 13, it was severe. Men, smokers, and older patients had more severe stages. Patients using higher doses of corticosteroids had lower severity of periodontitis. No relationship between the severity of periodontitis and AR activity was found. Conclusions: Periodontitis is common and severe in patients with RA, and is influenced by gender, age, smoking and corticosteroid dose.

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