Indirect immunofluorescence assay for the simultaneous detection of antibodies against clinically important old and new world hantaviruses

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dc.contributor.authorLederer, Sabine
dc.contributor.authorLattwein, Erik
dc.contributor.authorHanke, Merle
dc.contributor.authorSonnenberg, Karen
dc.contributor.authorStoecker, Winfried
dc.contributor.authorLundkvist, Ake
dc.contributor.authorVaheri, Antti
dc.contributor.authorVapalahti, Olli
dc.contributor.authorChan, Paul
dc.contributor.authorFeldmann, Heinz
dc.contributor.authorDick, Daryl
dc.contributor.authorSchmidt-Chanasit, Jonas
dc.contributor.authorPadula, Paula
dc.contributor.authorVial, Pablo
dc.contributor.authorPanculescu-Gatej, Raluca
dc.contributor.authorCeianu, Cornelia
dc.contributor.authorHeyman, Paul
dc.contributor.authorAvsic-Zupanc, Tatjana
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2017-04-18T14:53:13Z
dc.date.available2017-04-18T14:53:13Z
dc.date.issued2013
dc.description.abstractIn order to detect serum antibodies against clinically important Old and New World hantaviruses simultaneously, multiparametric indirect immunofluorescence assays (IFAs) based on biochip mosaics were developed. Each of the mosaic substrates consisted of cells infected with one of the virus types Hantaan (HTNV), Puumala (PUUV), Seoul (SEOV), Saaremaa (SAAV), Dobrava (DOBV), Sin Nombre (SNV) or Andes (ANDV). For assay evaluation, serum IgG and IgM antibodies were analyzed using 184 laboratory-confirmed hantavirus-positive sera collected at six diagnostic centers from patients actively or previously infected with the following hantavirus serotypes: PUUV (Finland, n=97); SEOV (China, n=5); DOBV (Romania, n=7); SNV (Canada, n=23); ANDV (Argentina and Chile, n=52). The control panel comprised 89 sera from healthy blood donors. According to the reference tests, all 184 patient samples were seropositive for hantavirus-specific IgG (n=177; 96%) and/or IgM (n=131; 72%), while all control samples were tested negative. In the multiparametric IFA applied in this study, 183 (99%) of the patient sera were IgG and 131 (71%) IgM positive (accordance with the reference tests: IgG, 96%; IgM, 93%). Overall IFA sensitivity for combined IgG and IgM analysis amounted to 100% for all serotypes, except for SNV (96%). Of the 89 control sera, 2 (2%) showed IgG reactivity against the HTNV substrate, but not against any other hantavirus. Due to the high cross-reactivity of hantaviral nucleocapsid proteins, endpoint titrations were conducted, allowing serotype determination in >90% of PUUV- and ANDV-infected patients. Thus, multiparametric IFA enables highly sensitive and specific serological diagnosis of hantavirus infections and can be used to differentiate PUUV and ANDV infection from infections with Murinae-borne hantaviruses (e.g. DOBV and SEOV).
dc.format.extent9
dc.identifier.citationPLoS Negl Trop Dis. 2013 Apr 4;7(4):e2157
dc.identifier.urihttp://hdl.handle.net/11447/1170
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pntd.0002157
dc.language.isoen_US
dc.publisherPLoS
dc.subjectAntibodies, Viral/analysis
dc.subjectAntibodies, Viral/immunology
dc.subjectFluorescent Antibody Technique, Indirect/methods
dc.subjectHantavirus/immunology
dc.titleIndirect immunofluorescence assay for the simultaneous detection of antibodies against clinically important old and new world hantaviruses
dc.typeArtículo

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