Intraarticular administration of dexamethasone after mesenchymal stem cells implantation does not improve significantly the treatment of preestablished full-thickness chondral defect in a rabbit model
Date
2013
Type:
Artículo
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9
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Journal Title
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Volume Title
Publisher
Sage Publications
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Abstract
Objective: The aim of this study was to evaluate the contribution to hyaline cartilage regeneration of dexamethasone
intraarticular administration after autologous mesenchymal stem cells (MSCs) implantation into a preestablished knee
full-thickness chondral defect. Design: Full-thickness chondral defects of 4.5 × 4.5 mm2
were surgically made in both medial
femoral condyles of adult male New Zealand rabbits. Two weeks later, autologous ex vivo expanded bone marrow–derived
MSCs were embedded in hyaluronic acid and implanted into the chondral defects. Immediately and every week after the
intervention, dexamethasone 0.25 mg/kg was intraarticularly administered (MSC/dexa-treated group). Six weeks after MSC
transplantation, the animals were euthanized and condyles were characterized molecularly according to aggrecan, collagen
type II, and collagen type I gene expression (quantitative reverse transcriptase-polymerase chain reaction) and histologically
(hematoxylin–eosin staining). Data of MSC/dexa-treated condyles were compared with untreated, dexa-treated, MSCtreated,
or normal unlesioned condyles. Results: The ratio between collagen type II expression versus collagen type I
expression in MSC/dexa-treated condyles was higher than one, even though the group mean value was not statistically
different from that of untreated defects. Histological changes were observed between MSC/dexa-treated and untreated
defects mainly in surface regularity and in hyaline matrix abundance. However, International Cartilage Repair Society score
analysis did not support robust differences between those groups. Conclusion: Intraarticular administration of dexamethasone after autologous MSC implantation into a preestablished full-thickness chondral defect does not contribute significantly to
the regeneration of a tissue with molecular and histological characteristics identical to hyaline cartilage.
Description
Centro de Medicina Regenerativa
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Citation
Cartilage. 2013 Apr; 4(2): 144–152
Keywords
hyaline cartilage regeneration, full-thickness chondral defect, mesenchymal stem cells, dexamethasone