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¿Es posible utilizar las muestras de colecistectomías con cáncer en investigación?: Calidad del ADN de muestras obtenidas del sistema público y privado de salud

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dc.contributor.author Roa, Iván
dc.contributor.author De Toro, Gonzalo
dc.contributor.author Sánchez, Tamara
dc.contributor.author Slater, Jeannie
dc.contributor.author Ziegler, Anne Marie
dc.contributor.author Game, Anakaren
dc.contributor.author Arellano, Leonardo
dc.contributor.author Schalper, Kurt
dc.contributor.author De Aretxabala, Xabier
dc.date.accessioned 2017-03-03T13:17:01Z
dc.date.available 2017-03-03T13:17:01Z
dc.date.issued 2013
dc.identifier.citation Revista Médica de Chile 2013, vol.141, p.1528-1533 es_CL
dc.identifier.uri http://dx.doi.org/10.4067/S0034-98872013001200005 es_CL
dc.identifier.uri http://hdl.handle.net/11447/995
dc.description.abstract Background: The quality of the archival samples stored at pathology services could be a limiting factor for molecular biology studies. Aim: To determine the quality of DNA extracted from gallbladder cancer samples at different institutions. Material and Methods: One hundred ninety four samples coming from five medical centers in Chile, were analyzed. DNA extraction was quantified determining genomic DNA concentration. The integrity of DNA was determined by polymerase chain reaction amplification of different length fragments of a constitutive gene (β-globin products of 110, 268 and 501 base pairs). Results: The mean DNA concentration obtained in 194 gallbladder cancer samples was 48 ± 43.1 ng/µl. In 22% of samples, no amplification was achieved despite obtaining a mean DNA concentration of 58.3 ng/ul. In 81, 67 and 22% of samples, a DNA amplification of at least 110, 268 or 501 base pairs was obtained, respectively. No differences in DNA concentration according to the source of the samples were demonstrated. However, there were marked differences in DNA integrity among participating centers. Samples from public hospitals were of lower quality than those from private clinics. Conclusions: Despite some limitations, in 80% of cases, the integrity of DNA in archival samples from pathology services in our country would allow the use of molecular biology techniques. es_CL
dc.format.extent 6 es_CL
dc.language.iso spa es_CL
dc.publisher Sociedad Médica de Santiago es_CL
dc.subject DNA neoplasm es_CL
dc.subject Gallbladder neoplasms es_CL
dc.subject Molecular biology es_CL
dc.title ¿Es posible utilizar las muestras de colecistectomías con cáncer en investigación?: Calidad del ADN de muestras obtenidas del sistema público y privado de salud es_CL
dc.title.alternative Quality of DNA from archival pathological samples of gallbladder cancer es_CL
dc.type Artículo es_CL


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