¿Es posible utilizar las muestras de colecistectomías con cáncer en investigación?: Calidad del ADN de muestras obtenidas del sistema público y privado de salud
Date
2013
Type:
Artículo
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6
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ORCID:
Journal Title
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Volume Title
Publisher
Sociedad Médica de Santiago
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Abstract
Background: The quality of the archival samples stored at pathology services could
be a limiting factor for molecular biology studies. Aim: To determine the quality of
DNA extracted from gallbladder cancer samples at different institutions. Material
and Methods: One hundred ninety four samples coming from five medical centers
in Chile, were analyzed. DNA extraction was quantified determining genomic DNA
concentration. The integrity of DNA was determined by polymerase chain reaction
amplification of different length fragments of a constitutive gene (β-globin products of
110, 268 and 501 base pairs). Results: The mean DNA concentration obtained in 194
gallbladder cancer samples was 48 ± 43.1 ng/µl. In 22% of samples, no amplification
was achieved despite obtaining a mean DNA concentration of 58.3 ng/ul. In 81, 67
and 22% of samples, a DNA amplification of at least 110, 268 or 501 base pairs was
obtained, respectively. No differences in DNA concentration according to the source
of the samples were demonstrated. However, there were marked differences in DNA
integrity among participating centers. Samples from public hospitals were of lower
quality than those from private clinics. Conclusions: Despite some limitations, in
80% of cases, the integrity of DNA in archival samples from pathology services in
our country would allow the use of molecular biology techniques.
Description
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Citation
Revista Médica de Chile 2013, 141:1528-1533
Keywords
DNA neoplasm, Gallbladder neoplasms, Molecular biology