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Human renal adipose tissue induces the invasion and progression of renal cell carcinoma

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dc.contributor.author Campo-Verde, Fiorella
dc.contributor.author López, José
dc.contributor.author Romeo, Leonardo
dc.contributor.author Giorlando, Noelia
dc.contributor.author Bruna, Flavia
dc.contributor.author Contador, David
dc.contributor.author López, Gastón
dc.contributor.author Santiano, Flavia
dc.contributor.author Sasso, Corina
dc.contributor.author Zyla, Leila
dc.contributor.author López, Constanza
dc.contributor.author Calvo, Juan
dc.contributor.author Carón, Rubén
dc.contributor.author Pistone, Virginia
dc.date.accessioned 2018-01-18T17:22:04Z
dc.date.available 2018-01-18T17:22:04Z
dc.date.issued 2017
dc.identifier.citation Oncotarget. 2017 Nov 7; 8(55): 94223–94234 es_CL
dc.identifier.uri http://dx.doi.org/10.18632/oncotarget.21666 es_CL
dc.identifier.uri http://hdl.handle.net/11447/1923
dc.description.abstract We evaluated the effects of conditioned media (CMs) of human adipose tissue from renal cell carcinoma located near the tumor (hRATnT) or farther away from the tumor (hRATfT), on proliferation, adhesion and migration of tumor (786-O and ACHN) and non-tumor (HK-2) human renal epithelial cell lines. Human adipose tissues were obtained from patients with renal cell carcinoma (RCC) and CMs from hRATnT and hRATfT incubation. Proliferation, adhesion and migration were quantified in 786-O, ACHN and HK-2 cell lines incubated with hRATnT-, hRATfT- or control-CMs. We evaluated versican, adiponectin and leptin expression in CMs from hRATnT and hRATfT. We evaluated AdipoR1/2, ObR, pERK, pAkt y pPI3K expression on cell lines incubated with CMs. No differences in proliferation of cell lines was found after 24 h of treatment with CMs. All cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRATnT-CMs vs. hRATfT- or control-CMs. hRATnT-CMs showed increased levels of versican and leptin, compared to hRATfT-CMs. AdipoR2 in 786-O and ACHN cells decreased significantly after incubation with hRATfT- and hRATnT-CMs vs. control-CMs. We observed a decrease in the expression of pAkt in HK-2, 786-O and ACHN incubated with hRATnT-CMs. This result could partially explain the observed changes in migration and cell adhesion. We conclude that hRATnT released factors, such as leptin and versican, could enhance the invasive potential of renal epithelial cell lines and could modulate the progression of the disease. es_CL
dc.format.extent 12 es_CL
dc.language.iso en_US es_CL
dc.publisher Impact Journals es_CL
dc.subject cancer es_CL
dc.subject epithelial-stromal interactions es_CL
dc.subject human adipose tissue es_CL
dc.subject migration es_CL
dc.subject renal epithelial cells es_CL
dc.title Human renal adipose tissue induces the invasion and progression of renal cell carcinoma es_CL
dc.type Artículo es_CL


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