Ulloa, María TeresaSanhueza, CamilaHenríquez, TaniaAguayo, BenjamínHermosilla, GermánPorte, LorenaDabanch, JeannetteBraun, StephanieFica, AlbertoBriceño, IsabelOsorio, Carlos2022-06-302022-06-302019Ulloa MT, Sanhueza C, Henríquez T, Aguayo B, Hermosilla G, Porte L, Dabanch J, Braun S, Fica A, Briceño I, Osorio CG. Cepas chilenas de origen clínico de Vibrio cholerae no-O1, no-O139 portan los genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF del sistema de secreción T3SS2 presentes en una isla de patogenicidad [Chilean strains of clinical origin of non-O1, non-O139 Vibrio cholerae carry the genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF from secretion system T3SS2 present in an island of pathogenicity]. Rev Chilena Infectol. 2019 Jun;36(3):312-317. Spanish. doi:10.4067/S0716-10182019000300312https://doi.org/10.4067/S0716-10182019000300312http://hdl.handle.net/11447/6282Backgound: The virulence factors of the Vibrio cholerae non-O1, non-O139 strains are not clearly known. The strain of septicemic origin NN1 Vibrio cholerae non-O1, non-O139 was sequenced previously by the Illumina platform. A fragment of the pathogenicity island VPaI-7 of V. parahaemolyticus was detected in its genome. Aim: To detect the virulence genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF in Chilean strains of V. cholerae non-O1, non-O139. Methods: A total of 9 Chilean strains of clinical origin of Vibrio cholerae non-O1, non-O139 isolated between 2006-2012 were analyzed by conventional PCR assays for type III secretion genes encoded on that island: vcsN2, vcsC2, vcsV2, vspD, toxR2 and vopF. Additionally, the presence of the virulence genes hylA and rtxA was determined. In addition, REP-PCR and ERIC-PCR assays were performed. Results: most (6/9) Chilean V. cholerae non-O1, non-O139 strains contain the type III secretion genes vcsN2, vcsC2, vcsV2, vspD, toxR2 and vopF, encoded in an island of pathogenicity. In addition, all (9/9) the strains contain the virulence genes hylA and rtxA. Conclusion: These results strongly suggest the possibility that those strains possess an important virulence potential in humans.esGastroenteritisVibrio cholerae non-O1Genomic islandsVirulence factorsT3SS2ChileVibrio cholerae no-O1Islas genómicasFactores de virulenciaArticle