Muñoz, PabloHernandez, Mauricio2025-03-032025-03-032024Entrepreneurship & Regional Development, 36(5–6), 577–606.https://hdl.handle.net/11447/9891In this paper, we explore the micro-interactions through which a regenerative enterprise engages with proximate natural ecosystems in its attempt to repair and protect them. Through an ethnographic study of a regenerative farming enterprise in rural Southern Patagonia - Fundo Panguilemu - we discover a reciprocal relationship between the enterprise and animals, central to their regenerative efforts. This relationship is formed and actively maintained by the founders through three practices – joint rewilding, ambivalent relationality, and task interdependence. We leverage nature relatedness to conceptualize the relationship between these practices as human-animal mutualism in regenerative work. We advance regenerative entrepreneurship research by revealing novel human-nature interactions formed and fostered by a rural enterprise in the pursuit of local regeneration and expand our understanding of micro-level phenomena in rural entrepreneurship.31 p.enRegenerative entrepreneurshipRural entrepreneurshipMutualismHuman-animal workRural PatagoniaNature relatednessArticlehttps://doi.org/10.1080/08985626.2024.2305648