Figueroa, DavidEspinosa, MaximilianoCalvo, RafaelScheu, MaximilianoVaisman, AlexGallegos, MarcelaConget, Paulette2017-03-212017-03-212014Knee Surgery, Sports Traumatology, Arthroscopy, 2014 ,22(5):1196-1202http://hdl.handle.net/11447/1046http://dx.doi.org/10.1007/s00167-013-2471-6Purpose: The objective of this study was to determine whether using mesenchymal stem cells (MSC) seeded in a collagen type I scaffold would be sufficient to regenerate the torn anterior cruciate ligament (ACL). Methods: Anterior cruciate ligament transection was performed on both knees in 10 New Zealand rabbits and then repaired with as follows: suture alone (suture-treated group, n = 6), suture associated with collagen type I scaffold (collagen type I scaffold-treated group, n = 8) or suture associated with autologous MSC seeded on collagen type I scaffold (MSC/collagen type I scaffold-treated group, n = 6). At 12-week post-intervention, the animals were killed and the ACLs were characterised macroscopically and histologically. Data of the 3 groups were against normal ACL (normal group, n = 10). Results: Macroscopic observation found that in MSC/collagen type I scaffold group, 33 % of specimens showed a complete ACL regeneration, with a tissue similar to the normal ACL. Regeneration was not observed in the group treated with suture alone or associated with collagen type I scaffold without cells. In the latter, only a reparative attempt at the ends was observed. Histological analysis of the regenerated ACL showed a tissue with organised collagen and peripheric vessels. Conclusions: These results provide evidence that the use of MSC seeded in a collagen type I scaffold in the treatment of ACL injuries is associated with an enhancement of ligament regeneration. This MSC-based technique is a potentially attractive tool for improving the treatment of ACL ruptures.7en-USAnterior cruciate ligamentMesenchymal stem cellsCollagen type I scaffoldRegenerationTissue engineeringArtículo