Ni, ZhenLeodori, GiorgioVial Undurraga, FelipeZhang, YongV. Avram, AlexandruPajevic, SinisaJ. Basser, PeterHallett, Mark2021-10-252021-10-252020Brain Stimulation 13 (2020) 1453e1460https://doi.org/10.1016/j.brs.2020.08.004http://hdl.handle.net/11447/4908Background: Neuroimaging technology is being developed to enable non-invasive mapping of the latency distribution of cortical projection pathways in white matter, and correlative clinical neurophysiological techniques would be valuable for mutual verification. Interhemispheric interaction through the corpus callosum can be measured with interhemispheric facilitation and inhibition using transcranial magnetic stimulation. Objective: To develop a method for determining the latency distribution of the transcallosal fibers with transcranial magnetic stimulation. Methods: We measured the precise time courses of interhemispheric facilitation and inhibition with a conditioning-test paired-pulse magnetic stimulation paradigm. The conditioning stimulus was applied to the right primary motor cortex and the test stimulus was applied to the left primary motor cortex. The interstimulus interval was set at 0.1 ms resolution. The proportions of transcallosal fibers with different conduction velocities were calculated by measuring the changes in magnitudes of interhemispheric facilitation and inhibition with interstimulus interval. Results: Both interhemispheric facilitation and inhibition increased with increment in interstimulus interval. The magnitude of interhemispheric facilitation was correlated with that of interhemispheric inhibition. The latency distribution of transcallosal fibers measured with interhemispheric facilitation was also correlated with that measured with interhemispheric inhibition. Conclusions: The data can be interpreted as latency distribution of transcallosal fibers. Interhemispheric interaction measured with transcranial magnetic stimulation is a promising technique to determine the latency distribution of the transcallosal fibers. Similar techniques could be developed for other cortical pathways.enCorpus callosumInterhemispheric facilitation and interhemispheric inhibitionLatency distributionMotor evoked potentialPrimary motor cortexTranscranial magnetic stimulationArticle