Wozniak, AnielaFigueroa, CristianMoya-Flores, FranciscoGuggiana, PieroCastillo, ClaudiaRivas, LinaMunita, JoséGarcía, Patricia C.2022-01-112022-01-112021Wozniak, A., Figueroa, C., Moya-Flores, F. et al. A multispecies outbreak of carbapenem-resistant bacteria harboring the blaKPC gene in a non-classical transposon element. BMC Microbiol 21, 107 (2021). https://doi.org/10.1186/s12866-021-02169-3https://doi.org/10.1186/s12866-021-02169-3http://hdl.handle.net/11447/5429Klebsiella pneumoniae is the most frequent KPC-producing bacteria. The blaKPC gene is frequently embedded in Tn4401 transposon, and less frequently in non-Tn4401 elements (NTEKPC) variants I-III. The first case of KPC in the UC-CHRISTUS Clinical Hospital was detected in Pseudomonas aeruginosa. Soon after this event, KPC was detected in 2 additional Pseudomonas aeruginosa, 3 Escherichia coli, 3 Enterobacter cloacae, 3 Klebsiella pneumoniae, and 1 Citrobacter freundii, isolated from 6 different patients. We aimed to elucidate the possible mechanisms of genetic transfer and dissemination of the blaKPC gene among isolates of this multispecies outbreak. A molecular epidemiology analysis of the above mentioned clinical isolates (n = 13) through Multi-Locus Sequence Typing, plasmid analysis, Pulsed-Field Gel-Electrophoresis, and Whole-genome sequencing (WGS) was performed.esKPC CarbapenemaseCarbapenem resistant EnterobacteriaceaeMultispecies outbreakHorizontal gene transferArticle