Munita, JoséTran, TrucDiaz, LorenaPanesso, DianaReyes, JinnetheMurray, BarbaraArias, Cesar2017-04-182017-04-182013Antimicrobial Agents Chemotherapy, 2013,57(6):2831-3http://hdl.handle.net/11447/1164http://dx.doi.org/10.1128/AAC.00021-13The genetic bases for antibiotic tolerance are obscure. Daptomycin (DAP) is a lipopeptide antibiotic with bactericidal activity against enterococci. Using time-kill assays, we provide evidence for the first time that a deletion of isoleucine in position 177 of LiaF, a member of the three-component regulatory system LiaFSR involved in the cell envelope response to antimicrobials, is directly responsible for a DAP-tolerant phenotype and is likely to negatively affect response to DAP therapy.3en-USBacterial Proteins/geneticsCodon/geneticsDaptomycin/pharmacologyDrug Resistance, Bacterial/geneticsEnterococcus faecium/drug effectsSequence Deletion/geneticsA liaF codon deletion abolishes daptomycin bactericidal activity against vancomycin-resistant enterococcus faecaliArtÃculo