Salgado-Caxito, MaríliaBenavides, Julio A.Munita, JoséRivas, LinaGarcía, PatriciaListoni, Fernando J. P.Paes, Antonio C.Moreno-Switt, Andrea I.2022-01-112022-01-112021Salgado-Caxito M, Benavides JA, Munita JM, Rivas L, García P, Listoni FJP, Moreno-Switt AI, Paes AC. Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil. Prev Vet Med. 2021 May;190:105316. doi: 10.1016/j.prevetmed.2021.105316https://doi.org/10.1016/j.prevetmed.2021.105316http://hdl.handle.net/11447/5419Faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli (ESC-R E. coli) in dogs has been reported worldwide and can reduce the effectiveness of treatments against bacterial infections. However, the drivers that influence faecal carriage of ESC-R E. coli in dogs are poorly understood. The aims of this study were to estimate the prevalence of ESC-R E. coli among dogs prior to their admission to a veterinary teaching hospital and to identify risk factors associated with the faecal carriage of ESC-R E. coli. Rectal swabs (n = 130) were collected from dogs and screened for ESC-R E. coli using MacConkey agar supplemented with cefotaxime (2 μg/mL). E. coli species was confirmed by MALDI-TOF and screening of extended-spectrum beta-lactamase (ESBL) genes was conducted by multiplex PCR. Questionnaires were completed by each dog's owner to test several human and dog characteristics associated with ESC-R E. coli. The prevalence of faecal carriage of ESC-R E. coli was 9.2 % and 67 % of ESC-R E. coli isolates harboured ESBL genes including CTX-M alone or in combination with TEM. All ESC-R E. coli isolates were resistant to ceftriaxone, cefpodoxime, and cefotaxime and were susceptible to cefoxitin and carbapenems. The likelihood of carrying ESC-R E. coli was 15 times higher (OR = 14.41 [95 % CI: 1.80-38.02], p < 0.01) if the dog was treated with antibiotics 3-12 months prior to sampling and 8 times higher (OR = 7.96 [95 % CI: 2.96-92.07], p < 0.01) if the dog had direct contact with livestock, but 15 times lower (OR = 0.07 [95 % CI: 0.01-0.32], p < 0.01) if the dog was dewormed during the previous year. Our findings confirm the faecal carriage of ESC-R E. coli in subclinical dogs and call for further investigation regarding the impact of deworming on antibiotic-resistant bacteria in companion animals.enAnti-Bacterial Agents / pharmacologyBrazil / epidemiologyCarrier State / microbiologyCephalosporins / pharmacologyDog Diseases / drug therapyDog Diseases / epidemiologyDog Diseases / microbiologyDrug Resistance, BacterialEscherichia coli / drug effectsEscherichia coli Infections / drug therapyEscherichia coli Infections / epidemiologyArticle