Hernandez, MariaRembao, JesusHernandez-Baltazar, DanielCastillo-Rodriguez, RosaTellez-Lopez, VictorFlores-Martinez, Yazmin2017-03-132017-03-132014Nanomedicine. 2014 May;10(4):745-54http://hdl.handle.net/11447/1020http://dx.doi.org/10.1016/j.nano.2013.11.013Centro de Medicina RegenerativaNeurotensin (NTS)-polyplex is a gene nanocarrier that has potential nanomedicine-based applications for the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. We assessed the acute inflammatory response to NTS-polyplex carrying a reporter gene in BALB/c mice. The intravenous injection of NTS-polyplex caused the specific expression of the reporter gene in gastrointestinal cells. Six hours after an intravenous injection of propidium iodide labeled-NTS-polyplex, fluorescent spots were located in the cells of the organs with a mononuclear phagocyte system, suggesting NTS-polyplex clearance. In contrast to lipopolysaccharide and carbon tetrachloride, NTS-polyplex did not increase the serum levels of tumor necrosis factor alpha, interleukin (IL)-1β, IL-6, bilirubin, aspartate transaminase, and alanine transaminase. NTS-polyplex increased the levels of serum amyloid A and alkaline phosphatase, but these levels normalized after 24 h. Compared to carrageenan, the local injection of NTS-polyplex did not produce inflammation. Our results support the safety of NTS-polyplex.10en-USBiosafetyCytotoxicityNanomaterialsPro-inflammatory cytokinesArtículo