Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma
Date
2019
Type:
Article
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ORCID:
Journal Title
Journal ISSN
Volume Title
Publisher
Impact Journals, LLC
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Abstract
Tumor cells can interact with neighboring adipose tissue. We evaluated
components present in human adipose explants from normal (hRAN) and kidney
cancer (hRAT) tissue, and we evaluated the effects of conditioned media (CMs)
from hRAN and hRAT on proliferation, adhesion and migration of tumor and nontumor
human renal epithelial cell lines. In addition, we evaluated the expression of
AdipoR1, ObR, CD44, vimentin, pERK and pPI3K on cell lines incubated with CMs.
hRAN were obtained from healthy operated donors, and hRAT from patients who
underwent a nephrectomy. hRAT showed increased levels of versican, leptin and ObR;
and decreased levels of perilipin, adiponectin and AdipoR1, compared to hRAN. Cell
lines showed a significant decrease in cell adhesion and increase in cell migration
after incubation with hRAT-CMs vs. hRAN- or control-CMs. Surprisingly, HK-2, 786-
O and ACHN cells showed a significant decrease in cell migration after incubation
with hRAN-CMs vs. control-CMs. No difference in proliferation of cell lines was found
after 24 or 48 h of treatment with CMs. AdipoR1 in ACHN and Caki-1 cells decreased
significantly after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. ObR and
CD44 increased in tumor line cells, and vimentin increased in non-tumor cells, after
incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. We observed an increase
in the expression of pERK and pPI3K in HK-2, 786-O and ACHN, incubated with hRATCMs.
In conclusion, results showed that adipose microenvironment can regulate the
behavior of tumor and non tumor human renal epithelial cells.
Description
Centro de Medicina Regenerativa (ICIM)
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Citation
Oncotarget, 2019, Vol. 10, (No. 52), pp: 5454-5467
Keywords
Human renal adipose tissue, Renal epithelial cells, Cancer, Epithelial-stromal interactions, Migration