Institutional Repository UDD

Administration of multipotent mesenchymal stromal cells restores liver regeneration and improves liver function in obese mice with hepatic steatosis after partial hepatectomy

Show simple item record

dc.contributor.author Ezquer, Fernando
dc.contributor.author Bahamonde, Javiera
dc.contributor.author Huang, Ya-Lin
dc.contributor.author Ezquer, Marcelo
dc.date.accessioned 2017-10-02T15:06:15Z
dc.date.available 2017-10-02T15:06:15Z
dc.date.issued 2017
dc.identifier.citation Stem Cell Res Ther. 2017; 8: 20
dc.identifier.uri http://hdl.handle.net/11447/1684
dc.identifier.uri http://dx.doi.org/10.1186/s13287-016-0469-y
dc.description.abstract Background: The liver has the remarkable capacity to regenerate in order to compensate for lost or damaged hepatic tissue. However, pre-existing pathological abnormalities, such as hepatic steatosis (HS), inhibits the endogenous regenerative process, becoming an obstacle for liver surgery and living donor transplantation. Recent evidence indicates that multipotent mesenchymal stromal cells (MSCs) administration can improve hepatic function and increase the potential for liver regeneration in patients with liver damage. Since HS is the most common form of chronic hepatic illness, in this study we evaluated the role of MSCs in liver regeneration in an animal model of severe HS with impaired liver regeneration. Methods: C57BL/6 mice were fed with a regular diet (normal mice) or with a high-fat diet (obese mice) to induce HS. After 30 weeks of diet exposure, 70% hepatectomy (Hpx) was performed and normal and obese mice were divided into two groups that received 5 × 105 MSCs or vehicle via the tail vein immediately after Hpx. Results: We confirmed a significant inhibition of hepatic regeneration when liver steatosis was present, while the hepatic regenerative response was promoted by infusion of MSCs. Specifically, MSC administration improved the hepatocyte proliferative response, PCNA-labeling index, DNA synthesis, liver function, and also reduced the number of apoptotic hepatocytes. These effects may be associated to the paracrine secretion of trophic factors by MSCs and the hepatic upregulation of key cytokines and growth factors relevant for cell proliferation, which ultimately improves the survival rate of the mice. Conclusions: MSCs represent a promising therapeutic strategy to improve liver regeneration in patients with HS as well as for increasing the number of donor organs available for transplantation.
dc.format.extent 18
dc.language.iso en_US
dc.publisher BioMed Central
dc.subject Hepatic steatosis
dc.subject Liver regeneration
dc.subject Multipotent mesenchymal stromal cells
dc.subject Mesenchymal stem cells
dc.subject Regenerative medicine
dc.title Administration of multipotent mesenchymal stromal cells restores liver regeneration and improves liver function in obese mice with hepatic steatosis after partial hepatectomy
dc.type Artículo


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account