Institutional Repository UDD

Temperature dependence of bilayer properties in liposomes and the use of fluorescent probes as a tool to elucidate the permeation mechanism of hydrophilic solutes

Show simple item record

dc.contributor.author Ahumada, M
dc.contributor.author Calderon, C
dc.contributor.author Lissie, A
dc.date.accessioned 2017-12-28T18:22:24Z
dc.date.available 2017-12-28T18:22:24Z
dc.date.issued 2016
dc.identifier.citation J. Chil. Chem. Soc., 61, Nº 3 (2016) es_CL
dc.identifier.uri http://dx.doi.org/10.4067/S0717-97072016000300009 es_CL
dc.identifier.uri http://hdl.handle.net/11447/1802
dc.description.abstract Solute transport across lipidic membranes is a fundamental process for both living organisms and drug delivery. In order to establish the mechanism of solute passive transport through lipidic membranes, we determined the effect of temperature upon the rate of glucose transport (as hydrophilic solute model) through dipalmitoylphosphatidylcholine large unilamellar liposomes and compare the results to those obtained for a variety of fluorescent probes (pyrene, PRODAN, diphenylhexatriene, diphenylhexatriene-TMA). All these probes, independent of their localization in the liposomes, report a monotonous change in the micro-properties sensed with temperature, with a maximum rate of change at the main transition temperature of the bilayer. These results contrast with those obtained for the rate of glucose influx, were it is observed a clear maximum rate of intake at the transition temperature. These contrasting results imply that the microviscosity (common factor for used probes) is not the property of the bilayer that controls the rate off solute transport which, at least in the vicinity of the main transition temperature, is regulated by formation of transient pores, particularly at the transition temperature (Tm). This would indicate that, at least near Tm, glucose and other hydrophilic solutes diffuses through transient pores formed in the bilayer. Interestingly, the presence of these pores does not affect the fluorescence characteristics of the probes. es_CL
dc.format.extent 3 es_CL
dc.language.iso en_US es_CL
dc.publisher Sociedad Chilena de Química es_CL
dc.subject liposome es_CL
dc.subject permeation es_CL
dc.subject glucose es_CL
dc.subject glucose oxidase es_CL
dc.subject fluorescent probe es_CL
dc.title Temperature dependence of bilayer properties in liposomes and the use of fluorescent probes as a tool to elucidate the permeation mechanism of hydrophilic solutes es_CL
dc.type Artículo es_CL


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account