Institutional Repository UDD

Photochemically induced fluorescence coupled to second-order multivariate calibration as analytical tool for determining imidacloprid in honeybees

Show simple item record

dc.contributor.author Jeria, Yanara
dc.contributor.author Bazaes, Aliosha
dc.contributor.author Baez, Maria E
dc.contributor.author Espinoza, Jeanette
dc.contributor.author Martinez, Jessica
dc.contributor.author Fuentes, Edwar
dc.date.accessioned 2017-09-11T15:23:26Z
dc.date.available 2017-09-11T15:23:26Z
dc.date.issued 2017
dc.identifier.citation Chem Int Lab Sys, Vol 160, 15 January 2017, Pages 1-7 es_CL
dc.identifier.uri http://dx.doi.org/10.1016/j.chemolab.2016.11.001 es_CL
dc.identifier.uri http://hdl.handle.net/11447/1664
dc.description.abstract This paper presents a method for the determination of imidacloprid in honeybees based on the measurement of excitation-emission spectra of photo-induced fluorescence (PIF-EEMs) associated to unfolded partial least squares coupled to residual bilinearization (U-PLS/RBL) algorithm. As a preliminary step, matrix solid phase dispersion (MSPD) using C18 as dispersant, combined on-line with a solid phase extraction (SPE) clean-up on graphitized carbon-amino propyl silica phase was applied to diminish the interferences presents in samples. A previous study on the photochemical induction of fluorescence of imidacloprid in presence of bee matrix was included. The second order advantage achieved with RBL permitted the determination of imidacloprid in the presence of interferences present in samples (unexpected compounds of bees), which also shows photo-induced or native fluorescence. The LOD was 20 µg kg−1 (2.5 ng per bee; four bees treated), which is suitable for detecting imidacloprid at the oral LD50 for the insect. The predicted U-PLS/RBL concentrations compared favorably with those measured using high-performance liquid chromatography with diode array detection. The PIF-EEMs coupled to U-PLS/RBL was applied for the determination of imidacloprid in honeybees collected in field hives. The work demonstrates the feasibility of the determination of imidacloprid in a highly complex sample matrix as bee through photochemically induced fluorescence spectroscopy coupled to multivariate calibration. es_CL
dc.format.extent 7 es_CL
dc.language.iso en_US es_CL
dc.publisher Elsevier es_CL
dc.subject Imidacloprid es_CL
dc.subject Honeybees es_CL
dc.subject Photo-induced fluorescence es_CL
dc.subject Multivariate calibration es_CL
dc.title Photochemically induced fluorescence coupled to second-order multivariate calibration as analytical tool for determining imidacloprid in honeybees es_CL
dc.type Artículo es_CL


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account