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Impact of changing from staining to culture techniques on detection rates of campylobacter spp. in routine stool samples in Chile

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dc.contributor.author Porte, Lorena
dc.contributor.author Varela, Carmen
dc.contributor.author Haecker, Thomas
dc.contributor.author Morales, Sara
dc.contributor.author Weitzel, Thomas
dc.date.accessioned 2017-05-10T13:06:29Z
dc.date.available 2017-05-10T13:06:29Z
dc.date.issued 2016
dc.identifier.citation BMC Infect Dis. 2016; 16: 196 es_CL
dc.identifier.uri http://dx.doi.org/10.1186/s12879-016-1546-7 es_CL
dc.identifier.uri http://hdl.handle.net/11447/1221
dc.description.abstract Background Campylobacter is a leading cause of bacterial gastroenteritis, but sensitive diagnostic methods such as culture are expensive and often not available in resource limited settings. Therefore, direct staining techniques have been developed as a practical and economical alternative. We analyzed the impact of replacing Campylobacter staining with culture for routine stool examinations in a private hospital in Chile. Methods From January to April 2014, a total of 750 consecutive stool samples were examined in parallel by Hucker stain and Campylobacter culture. Isolation rates of Campylobacter were determined and the performance of staining was evaluated against culture as the gold standard. Besides, isolation rates of Campylobacter and other enteric pathogens were compared to those of past years. Results Campylobacter was isolated by culture in 46 of 750 (6.1 %) stool samples. Direct staining only identified three samples as Campylobacter positive and reached sensitivity and specificity values of 6.5 and 100 %, respectively. In comparison to staining-based detection rates of previous years, we observed a significant increase of Campylobacter cases in our patients. Conclusion Direct staining technique for Campylobacter had a very low sensitivity compared to culture. Staining methods might lead to a high rate of false negative results and an underestimation of the importance of campylobacteriosis. With the inclusion of Campylobacter culture, this pathogen became a leading cause of intestinal infection in our patient population. es_CL
dc.format.extent 6 es_CL
dc.language.iso en_US es_CL
dc.publisher BioMed Central es_CL
dc.subject Campylobacter es_CL
dc.subject Diagnosis es_CL
dc.subject Culture media es_CL
dc.subject Staining methods es_CL
dc.title Impact of changing from staining to culture techniques on detection rates of campylobacter spp. in routine stool samples in Chile es_CL
dc.type Artículo es_CL


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