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Cardiac stress test induced by dobutamine and monitored by cardiac catheterization in mice

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dc.contributor.author Calligaris, Sebastian
dc.contributor.author Ricca, Micaela
dc.contributor.author Conget, Paulette
dc.date.accessioned 2017-04-10T11:34:48Z
dc.date.available 2017-04-10T11:34:48Z
dc.date.issued 2013
dc.identifier.citation J Vis Exp. 2013 Feb 10;(72). pii: 50050 es_CL
dc.identifier.uri http://dx.doi.org/10.3791/50050 es_CL
dc.identifier.uri http://hdl.handle.net/11447/1116
dc.description Centro de Medicina Regenerativa es_CL
dc.description.abstract Dobutamine is a β-adrenergic agonist with an affinity higher for receptor expressed in the heart (β1) than for receptors expressed in the arteries (β2). When systemically administered, it increases cardiac demand. Thus, dobutamine unmasks abnormal rhythm or ischemic areas potentially at risk of infarction. Monitoring of heart function during a cardiac stress test can be performed by either echocardiography or cardiac catheterization. The latter is an invasive but more accurate and informative technique that the former. Cardiac stress test induced by dobutamine and monitored by cardiac catheterization accomplished as described here allows, in a single experiment, the measurement of the following hemodynamic parameters: heart rate (HR), systolic pressure, diastolic pressure, end-diastolic pressure, maximal positive pressure development (dP/dtmax) and maximal negative pressure development (dP/dtmin), at baseline conditions and under increasing doses of dobutamine. As expected, in normal mice we observed a dobutamine dose-related increase in HR, dP/dtmax and dP/dtmin. Moreover, at the highest dose tested (12 ng/g/min) the cardiac decompensation of high fat diet-induced obese mice was unmasked. es_CL
dc.format.extent 6 es_CL
dc.language.iso en_US es_CL
dc.publisher MYJoVE Corporation es_CL
dc.subject Adrenergic beta-1 Receptor Agonists/administration & dosage es_CL
dc.subject Cardiac Catheterization/methods es_CL
dc.subject Dobutamine/administration & dosage es_CL
dc.subject Exercise Test/methods es_CL
dc.subject Heart/drug effects es_CL
dc.subject Heart/physiology es_CL
dc.subject Mice, Inbred C57BL es_CL
dc.title Cardiac stress test induced by dobutamine and monitored by cardiac catheterization in mice es_CL
dc.type Artículo es_CL


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