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Programas de reforestación de bosque nativo y cambio climático en el marco de la Ley 20.283.: El caso del bosque esclerófilo
(Universidad del Desarrollo. Facultad de Derecho, 2021) Alvarez, Pedro; Leiva, Felipe
El programa de reforestación es producto de las obligaciones asumidas al momento de efectuar un Plan de Manejo de Corta y Reforestación de Bosque Nativo para Ejecutar Obras Civiles. Sin embargo, en la actualidad la Ley N° 20.283 sobre Recuperación de Bosque Nativo y Fomento Forestal (2008) no contempla consideraciones en torno al efecto del cambio climático en estas reforestaciones comprometiendo el establecimiento de nuevos bosques en el largo plazo. Dentro de esta problemática, resalta el artículo 21 de la Ley N° 20.283 y artículo 33 Reglamento del Decreto ley N° 701, de 1974, sobre fomento forestal (1980), como fuentes del problema y la solución, ya que contienen las disposiciones para la reforestación con el “mismo tipo forestal” y “preferentemente” en la misma provincia, respectivamente. Actualizar el marco normativo permitiría transformar los programas de reforestación en un instrumento de gestión ambiental que permitiría la adaptación y migración de los ecosistemas terrestres frente a los impactos del cambio climático, y reduciría el riesgo de sanciones administrativas a los titulares de proyectos. Lo anterior es particularmente urgente para el tipo forestal esclerófilo que ya presenta indicios de mortalidad producto de la última mega sequía y el cambio climático.
Chromosome-Mediated Colistin Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli: Mutation Analysis in the Light of Genetic Background
(2023) Riquelme, María; Martinez, Rodrigo; Brito, Bárbara; García, Patricia; Legarraga, Paulette; Wozniak, Aniela
Purpose: Colistin resistance mechanisms involving mutations in chromosomal genes associated with LPS modification are not completely understood. Mutations in genes coding for the MgrB regulator frequently account for colistin resistance in Klebsiella pneumoniae, whereas mutations in genes coding for PhoPQ and PmrAB are frequent in E. coli. Our aim was to perform a genetic analysis of chromosomal mutations in colistin-resistant (MIC ≥4 µg/mL) clinical isolates of K. pneumoniae (n = 8) and E. coli (n = 7) of different STs.
Methods: Isolates were obtained in a 3-year period in a university hospital in Santiago, Chile. Susceptibility to colistin, aminoglycosides, cephalosporins, carbapenems and ciprofloxacin was determined through broth microdilution. Whole genome sequencing was performed for all isolates and chromosomal gene sequences were compared with sequences of colistin-susceptible isolates of the same sequence types.
Results: None of the isolates carried mcr genes. Most of the isolates were susceptible to all the antibiotics analyzed. E. coli isolates were ST69, ST127, ST59, ST131 and ST14, and K. pneumoniae isolates were ST454, ST45, ST6293, ST380 and ST25. All the isolates had mutations in chromosomal genes analyzed. K. pneumoniae had mutations mainly in mgrB gene, whereas E. coli had mutations in pmrA, pmrB and pmrE genes. Most of the amino acid changes in LPS-modifying enzymes of colistin-resistant isolates were found in colistin-susceptible isolates of the same and/or different ST. Eleven of them were found only in colistin-resistant isolates.
Conclusion: Colistin resistance mechanisms depend on genetic background, and are due to chromosomal mutations, which implies a lower risk of transmission than plasmid-mediated genes. Colistin resistance is not associated with multidrug-resistance, nor to high-risk sequence types.
Changes in tobacco imagery and smokers' depiction in Spanish top-grossing films before and after the implementation of a comprehensive tobacco control policy in Spain
(2023) Feliu, Ariadna; Quintero, Alex; Peruga, Armando; Carnicer, Dolors; Antón, Laura; Rey, Juan; Fernández, Esteve
Introduction: As more restrictions on tobacco marketing communication are implemented, tobacco marketing has persisted through smoking in films. Our aims were to assess changes in tobacco imagery exposure in Spanish top-grossing films before and after the banning of tobacco advertising in Spain, and to determine whether the depiction of smoking characters has changed over the years.
Methods: A repeated cross-sectional study measured the tobacco content in the 10 Spanish top-grossing films in 2005, 2010 and 2015 (n=30) before and after a complete tobacco advertising ban. We conducted a descriptive and regression analysis of changes in tobacco impressions by year.
Results: The 30 films contained 1378 tobacco occurrences (90.2% positive for tobacco) with a median length of eight seconds onscreen. Total tobacco occurrences deemed positive for tobacco interests significantly increased in 2010 and 2015 compared to 2005. However, we observed decreased odds of tobacco brands appearances (OR=0.25; p<0.001) in 2010 and of implied tobacco use (OR=0.44; p=0.002), and tobacco brands appearances (OR=0.36; p<0.001) in 2015 compared to 2005. There was a change of pattern in the type of role smokers played from a leading role to a supporting one (p<0.001). The population reach of positive for tobacco occurrence in Spanish top-grossing films decreased from 15.9 (95% CI: 15.86-15.86) per 1000 spectators in 2005 to 0.8 (95% CI: 0.82-0.82) in 2015.
Conclusions: The implementation of a ban on complete tobacco product advertising was followed by a decrease in tobacco incidents across top-grossing Spanish films. Yet, exposure to smoking in films is still unacceptably high.
Antibiotic Consumption During the Coronavirus Disease 2019 Pandemic and Emergence of Carbapenemase-Producing Klebsiella pneumoniae Lineages Among Inpatients in a Chilean Hospital: A Time-Series Study and Phylogenomic Analysis
(2023) Allel, Kasim; Peters, Anne Sophie; Conejeros, José; Martínez, José; Spencer, Maria; Riquelme, Roberto; Rivas Jiménez, Lina María; Rojas, Pamela; Orellana, Cristian; García, Patricia; Araos Bralic, Rafael Ignacio; McGovern, Olivia; Patel, Twisha; Arias, Cesar; Lessa, Fernanda; Undurraga, Eduardo; Munita, Jose M.
Background: The impact of coronavirus disease 2019 (COVID-19) on antimicrobial use (AU) and resistance has not been well evaluated in South America. These data are critical to inform national policies and clinical care.
Methods: At a tertiary hospital in Santiago, Chile, between 2018 and 2022, subdivided into pre- (3/2018-2/2020) and post-COVID-19 onset (3/2020-2/2022), we evaluated intravenous AU and frequency of carbapenem-resistant Enterobacterales (CRE). We grouped monthly AU (defined daily doses [DDD]/1000 patient-days) into broad-spectrum β-lactams, carbapenems, and colistin and used interrupted time-series analysis to compare AU during pre- and post-pandemic onset. We studied the frequency of carbapenemase-producing (CP) CRE and performed whole-genome sequencing analyses of all carbapenem-resistant (CR) Klebsiella pneumoniae (CRKpn) isolates collected during the study period.
Results: Compared with pre-pandemic, AU (DDD/1000 patient-days) significantly increased after the pandemic onset, from 78.1 to 142.5 (P < .001), 50.9 to 110.1 (P < .001), and 4.1 to 13.3 (P < .001) for broad-spectrum β-lactams, carbapenems, and colistin, respectively. The frequency of CP-CRE increased from 12.8% pre-COVID-19 to 51.9% after pandemic onset (P < .001). The most frequent CRE species in both periods was CRKpn (79.5% and 76.5%, respectively). The expansion of CP-CRE harboring blaNDM was particularly noticeable, increasing from 40% (n = 4/10) before to 73.6% (n = 39/53) after pandemic onset (P < .001). Our phylogenomic analyses revealed the emergence of two distinct genomic lineages of CP-CRKpn: ST45, harboring blaNDM, and ST1161, which carried blaKPC.
Conclusions: AU and the frequency of CP-CRE increased after COVID-19 onset. The increase in CP-CRKpn was driven by the emergence of novel genomic lineages. Our observations highlight the need to strengthen infection prevention and control and antimicrobial stewardship efforts.
Annona cherimola Seed Extracts Trigger an Early Apoptosis Response and Selective Anticlonogenic Activity against the Human Gastric Carcinoma Cell Line SNU-1
(2023) Macuer, Johan; Giovagnoli, Claudia; Bernal, Giuliano; Lobos, Lorena; De la Fuente, Erwin; Araya, Michael; Ibáñez, Cristian
The aim of this study was to evaluate, for the first time, the antiproliferative, apoptotic and diminishing effects of the anchored growth-independent capacity of an ethanol macerate extract from the Annona cherimola seed (EMCHS) in the human gastric cancer cell line SNU-1. The cells treated with EMCHS (20 μg/mL) significantly reduced the capacity to form clones of the tumor cell. Moreover, 50 μg/mL of EMCHS extract induced apoptosis, as was shown by the Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins (Annonacinone and Annonacin) in the EMCHS, which could be largely responsible for its selective antiproliferative effect. The identification of fatty acids by GC-FID showed the presence of eight fatty acids, among which was, oleic acid, which has recognized activity as an adjuvant in antitumor treatments. Taken together, our results indicate that the EMCHS seems promising for use as a natural therapy against gastric cancer disease.
Squamous Cell CarcinomaDeveloped in a TrichilemmalCyst, an Incidental Finding
(2023) Perez-Wilson, Jaime; Coulon, Gabriela; Whittle, Carolina; Castro-Avila, Ana Cristina
A 61-year-old man with no previous cancer history attended the dermatology clinic with a three-year history of asymptomatic swelling on the posterior aspect of the left elbow. Physical examination revealed a subcutaneous nodule of 1cm in diameter, with no epidermal changes. A Doppler ultrasound was requested, which revealed a predominantly dermal hypodermal cystic lesion with hypoechogenic content, an echogenic rim and small echogenic foci compatible with an epidermal cyst, no solid inner nodule was depicted (Fig. 1). An excisional biopsy was performed, which revealed a trichilemmal-type follicular cyst with a 0.5mm thickness well-differentiated squamous cell carcinoma (SCC) in its wall (Fig. 2). It was presented to the oncology committee that suggested local extent of 1cm The new biopsy did not show signs of residual neoplasia. At six months of follow-up, the patient did not present clinical or dermoscopic signs of recurrence.
Administration of Secretome Derived from Human Mesenchymal Stem Cells Induces Hepatoprotective Effects in Models of Idiosyncratic Drug-Induced Liver Injury Caused by Amiodarone or Tamoxifen
(2023) Huang, Ya-Lin; De Gregorio, Cristian; Silva, Verónica; Elorza, Álvaro; Léniz, Patricio; Aliaga, Víctor; Maracaja, Vinicius; Budini, Mauricio; Ezquer, Fernando; Ezquer, Marcelo
Drug-induced liver injury (DILI) is one of the leading causes of acute liver injury. While many factors may contribute to the susceptibility to DILI, obese patients with hepatic steatosis are particularly prone to suffer DILI. The secretome derived from mesenchymal stem cell has been shown to have hepatoprotective effects in diverse in vitro and in vivo models. In this study, we evaluate whether MSC secretome could improve DILI mediated by amiodarone (AMI) or tamoxifen (TMX). Hepatic HepG2 and HepaRG cells were incubated with AMI or TMX, alone or with the secretome of MSCs obtained from human adipose tissue. These studies demonstrate that coincubation of AMI or TMX with MSC secretome increases cell viability, prevents the activation of apoptosis pathways, and stimulates the expression of priming phase genes, leading to higher proliferation rates. As proof of concept, in a C57BL/6 mouse model of hepatic steatosis and chronic exposure to AMI, the MSC secretome was administered endovenously. In this study, liver injury was significantly attenuated, with a decrease in cell infiltration and stimulation of the regenerative response. The present results indicate that MSC secretome administration has the potential to be an adjunctive cell-free therapy to prevent liver failure derived from DILI caused by TMX or AMI.
A Third Dose of SARS-CoV-2 mRNA Vaccine Improves Immune Response in Chronic Kidney Disease Patients
(2023) Poli Harlowe, María Cecilia; Vial Cox, María Cecilia; Rey, Emma; González, Natalia; Cortés, Lina; Hormazabal, Juan; Ramírez, Carolina; De la Cruz, Javiera; Ulloa, Camilo
Chronic kidney disease (CKD) patients have an increased risk of morbidity and mortality following SARS-CoV-2 infection. Vaccination in these patients is prioritized, and monitoring of the immune response is paramount to define further vaccination strategies. This prospective study included a cohort of 100 adult CKD patients: 48 with kidney transplant (KT) and 52 on hemodialysis without prior COVID-19. The patients were assessed for humoral and cellular immune responses after four months of an anti-SARS-CoV-2 primary two-dose vaccination scheme (CoronaVac or BNT162b2) and one month after a booster third dose of BNT162b2 vaccine. We identified poor cellular and humoral immune responses in the CKD patients after a primary vaccination scheme, and these responses were improved by a booster. Robust polyfunctional CD4+ T cell responses were observed in the KT patients after a booster, and this could be attributed to a higher proportion of the patients having been vaccinated with homologous BNT162b2 schemes. However, even after the booster, the KT patients exhibited lower neutralizing antibodies, attributable to specific immunosuppressive treatments. Four patients suffered severe COVID-19 despite three-dose vaccination, and all had low polyfunctional T-cell responses, underscoring the importance of this functional subset in viral protection. In conclusion, a booster dose of SARS-CoV-2 mRNA vaccine in CKD patients improves the impaired humoral and cellular immune responses observed after a primary vaccination scheme.
A Novel Gemcitabine-Resistant Gallbladder Cancer Model Provides Insights into Molecular Changes Occurring during Acquired Resistance
(2023) Vergara, Luis; Bizama, Carolina; Zhong, Jun; Buchegger, Kurt; Suárez, Felipe; Rosa, Lorena; Ili, Carmen; Weber, Helga; Obreque, Javiera; Espinoza, Karena; Repetto, Gabriela; Roa, Juan; Leal, Pamela; García, Patricia
Treatment options for advanced gallbladder cancer (GBC) are scarce and usually rely on cytotoxic chemotherapy, but the effectiveness of any regimen is limited and recurrence rates are high. Here, we investigated the molecular mechanisms of acquired resistance in GBC through the development and characterization of two gemcitabine-resistant GBC cell sublines (NOZ GemR and TGBC1 GemR). Morphological changes, cross-resistance, and migratory/invasive capabilities were evaluated. Then, microarray-based transcriptome profiling and quantitative SILAC-based phosphotyrosine proteomic analyses were performed to identify biological processes and signaling pathways dysregulated in gemcitabine-resistant GBC cells. The transcriptome profiling of parental and gemcitabine-resistant cells revealed the dysregulation of protein-coding genes that promote the enrichment of biological processes such as epithelial-to-mesenchymal transition and drug metabolism. On the other hand, the phosphoproteomics analysis of NOZ GemR identified aberrantly dysregulated signaling pathways in resistant cells as well as active kinases, such as ABL1, PDGFRA, and LYN, which could be novel therapeutic targets in GBC. Accordingly, NOZ GemR showed increased sensitivity toward the multikinase inhibitor dasatinib compared to parental cells. Our study describes transcriptome changes and altered signaling pathways occurring in gemcitabine-resistant GBC cells, which greatly expands our understanding of the underlying mechanisms of acquired drug resistance in GBC.
Reliability and acceptability of web-based administration of Spanish ages and stages questionnaires third edition
(2023) Schonhaut , Luisa; Armijo, Ivan; Rojas, Paula; Cabrera, Leonardo; Boisier, Roberto
To analyse the reliability of 6 age intervals of Spanishweb-based Ages and Stages Questionnaires Third Edition(WB-ASQ-3) and parents' acceptability, in a clinical practicecontext, during COVID-19 pandemic and lockdown in Chile.Cross-sectional study with a longitudinal component. Inter-nal consistency was analysed by Cronbach's alpha and Pear-son product–moment correlation, Pearson's correlationsbetween intervals questionnaires were analysed. Accept-ability was determined using a parental satisfaction survey.1061 children completed 2318 validated questionnaires.Scores were compared with normative sample, except forGross motor at 8 and 12 months, and Communication at12, 18 and 24 months, without differences to the previousChileans sample. In domain analysis, Cronbach's alpharanged from 0.36 to 0.80 and reached 0.74 to 0.81 for theoverall test. Strong positive correlation between the domainand overall score was obtained. Correlation between mostquestionnaires at different ages was positive and significant.Parental surveys show high satisfaction; parents werethankful for being able to monitor children's development.WB-ASQ-3 is a reliable and feasible screening system, par-ticularly when the test is taken as a whole, rather than by domains. It is remarkably high acceptability among parents,especially in complex times such as the lockdown and theCOVID-19 pandemic.
Whole-genome sequencing reveals changes in genomic diversity and distinctive repertoires of T3SS and T6SS effector candidates in Chilean clinical Campylobacter strains
(2023) Katz, Assaf; Porte, Lorena; Weitzel, Thomas; Varela, Carmen; Muñoz, Cristina; Ugalde, Juan; Grim, Christopher; González, Narjol; Blondel, Carlos; Bravo, Verónica
Campylobacter is the leading cause of bacterial gastroenteritis worldwide and an emerging and neglected pathogen in South America. This zoonotic pathogen colonizes the gastrointestinal tract of a wide range of mammals and birds, with poultry as the most important reservoir for human infections. Apart from its high morbidity rates, the emergence of resistant strains is of global concern. The aims of this work were to determine genetic diversity, presence of antimicrobial resistance determinants and virulence potential of Campylobacter spp. isolated from patients with acute gastrointestinal disease at 'Clinica Alemana', Santiago de Chile. The study considered the isolation of Campylobacter spp., from stool samples during a 20-month period (January 2020 to September 2021). We sequenced (NextSeq, Illumina) and performed an in-depth analysis of the genome sequences of 88 Campylobacter jejuni and 2 Campylobacter coli strains isolated from clinical samples in Chile. We identified a high genetic diversity among C. jejuni strains and the emergence of prevalent clonal complexes, which were not identified in our previous reports. While ~40% of strains harbored a mutation in the gyrA gene associated with fluoroquinolone resistance, no macrolide-resistance determinants were detected. Interestingly, gene clusters encoding virulence factors such as the T6SS or genes associated with long-term sequelae such as Guillain-Barré syndrome showed lineage-relatedness. In addition, our analysis revealed a high degree of variability regarding the presence of fT3SS and T6SS effector proteins in comparison to type strains 81-176, F38011, and NCTC 11168 and 488. Our study provides important insights into the molecular epidemiology of this emerging foodborne pathogen. In addition, the differences observed regarding the repertoire of fT3SS and T6SS effector proteins could have an impact on the pathogenic potential and transmissibility of these Latin American isolates, posing another challenge in characterizing the infection dynamics of this emergent and neglected bacterial pathogen.
Análisis e intervención de las prácticas de co-enseñanza entre docentes y profesionales del programa de integración escolar
(Universidad del Desarrollo. Facultad de Psicología, 2023) Gallardo Vargas, Fabián; Contreras Hernández, Fernando
La inclusión educativa avanza a nivel institucional desde la creación de los Programas de Integración en 2009 mediante el decreto 170 y la promulgación del decreto 83 en 2015 que oficializa la obligación de los establecimientos educacionales de apuntar a un Diseño Universal de los aprendizajes. Es en el contexto antes mencionado que la co-enseñanza ha adquirido protagonismo en el quehacer educativo de los establecimiento del pais. No obstante, este protagonismo, no necesariamente se traduce en su implantación efectiva dentro del aula, quedando en muchas ocasiones en una declaración de intención y principios, con tal de cumplir con los escasos lineamientos emitidos a nivel central. Por lo anterior es que resulta fundamental fomentar en los equipos de aula espacios de reflexión, como el planteado en este documento, el cual mediante un proceso de diagnóstico buscaba atender a los principales obstáculos para la implantación efectiva de la co-enseñanza, entre los cuales fue posible establecer la falta de lineamientos específicos y capacitaciones orientadas al desarrollo de habilidades prácticas, que les permitieran operacionalizar y organizar el trabajo colaborativo con tal de generar dinámicas de co-enseñanza que impacten en el aula. Es así como los equipos de aula construyeron e identificaron mecanismos y metodológicas que les permitan optimizar sus tiempos, mediante una planificación conjunta, distribución de roles y asignación de responsabilidades, además de incorporar la sistematizas y reflexión posterior para una mejora continua.
Maintenance of chronicity signatures in fibroblasts isolated from recessive dystrophic epidermolysis bullosa chronic wound dressings under culture conditions
(2023) De Gregorio, Cristian; Catalán, Evelyng; Garrido, Gabriel; Morandé, Pilar; Castillo, Jimena; Muñoz, Catalina; Cofré, Glenda; Huang, Ya-Lin; Cuadra, Bárbara; Murgas, Paola; Calvo, Margarita; Altermatt, Fernando; Joao, María; Palisson, Francis; South, Andrew; Ezquer, Marcelo; Ezquer, Marcelo; Fuentes, Ignacia
Background:
Recessive Dystrophic Epidermolysis Bullosa (RDEB) is a rare inherited skin disease caused by variants in the COL7A1 gene, coding for type VII collagen (C7), an important component of anchoring fibrils in the basement membrane of the epidermis. RDEB patients suffer from skin fragility starting with blister formation and evolving into chronic wounds, inflammation and skin fibrosis, with a high risk of developing aggressive skin carcinomas. Restricted therapeutic options are limited by the lack of in vitro models of defective wound healing in RDEB patients.
Results:
In order to explore a more efficient, non-invasive in vitro model for RDEB studies, we obtained patient fibroblasts derived from discarded dressings) and examined their phenotypic features compared with fibroblasts derived from non-injured skin of RDEB and healthy-donor skin biopsies. Our results demonstrate that fibroblasts derived from RDEB chronic wounds (RDEB-CW) displayed characteristics of senescent cells, increased myofibroblast differentiation, and augmented levels of TGF-β1 signaling components compared to fibroblasts derived from RDEB acute wounds and unaffected RDEB skin as well as skin from healthy-donors. Furthermore, RDEB-CW fibroblasts exhibited an increased pattern of inflammatory cytokine secretion (IL-1β and IL-6) when compared with RDEB and control fibroblasts. Interestingly, these aberrant patterns were found specifically in RDEB-CW fibroblasts independent of the culturing method, since fibroblasts obtained from dressing of acute wounds displayed a phenotype more similar to fibroblasts obtained from RDEB normal skin biopsies.
Conclusions:
Our results show that in vitro cultured RDEB-CW fibroblasts maintain distinctive cellular and molecular characteristics resembling the inflammatory and fibrotic microenvironment observed in RDEB patients’ chronic wounds. This work describes a novel, non-invasive and painless strategy to obtain human fibroblasts chronically subjected to an inflammatory and fibrotic environment, supporting their use as an accessible model for in vitro studies of RDEB wound healing pathogenesis. As such, this approach is well suited to testing new therapeutic strategies under controlled laboratory conditions.
Successful eradication rate of Helicobacter pylori with empirical antibiotic treatment in pediatric patients from a Tertiary Hospital
(2023) Darritchon Lama, Sofía; Díaz García, Diego; Toledo Complido, Marcela; Lucero, Yalda
Helicobacter pylori infection is a common condition that, in the long term, is associated with the development of peptic ulcer disease and eventually gastric cancer, which could be prevented with timely treatment. Optimally, eradication success should be greater than 90%, but the recommended empirical
reatments do not achieve these rates in real-life conditions. Objective: To determine the success rate of first-line empirical eradication treatment against H. pylori in pediatric patients treated in a tertiary hospital. Patients and Method: Retrospective descriptive study in patients with H. pylori infection detected in gastric biopsies and who had received first-line antibiotic treatment during the period 2017-2021. A negative result of an antigen test in stools or new
iopsies after ≥ 1 month after
Promoción de estrategias de autorregulación para el aprendizaje en estudiantes de técnico en nivel superior de educación diferencial en un Centro de Formación Técnica
(Universidad del Desarrollo. Facultad de Psicología, 2023) Bravo Garrido, Melisa; Hojman Ancelovici, Viviana
Este proyecto de intervención tuvo por propósito promover estrategias de autorregulación para el aprendizaje en estudiantes de Técnico en Nivel Superior de Educación Diferencial en el contexto de un Centro de Formación Técnica, mediante talleres formativos dirigidos a la comunidad estudiantil.
La metodología empleada para la elaboración del proyecto responde a un levantamiento de problemática inicial mediante un proceso de diagnóstico y una delimitación teórica, por medio de la realización de una revisión bibliográfica que se reporta como un marco referencial.
El proceso de diagnóstico en primera instancia quería evaluar las capacidades de autorregular el aprendizaje en contextos de clases remotas virtuales, no obstante, este foco se moviliza a evaluar las capacidades de aprendizaje autorregulado de forma independiente a la modalidad de clase, a partir de lo cual se levantan las acciones de la intervención.
Tras realizar el proceso de intervención se puede concluir un logro total de las acciones; sin embargo, no se logra evidenciar del mismo modo un logro de los objetivos de aprendizaje presupuestados en el taller formativo, dado que existió una limitante importante de asistencia en los talleres, contando con sólo el 25% de participantes inscritos.
Como reflexiones finales de este informe, se da cuenta que la participación de los talleres también depende de habilidades de autorregulación de los estudiantes, por lo que, al tratarse de una asistencia voluntaria, un escenario posible era no contar con la adherencia esperada. De este modo se finaliza este reporte, señalando posibles medidas que pudieran garantizar mayor participación de estudiantes en situaciones similares que contribuyan a formar sus habilidades de aprendizaje autorregulado.